The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels

doi:10.1038/s41438-021-00523-0

	The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels
	Wang, Weihao ; Wang, Peiwen 1; Li, Xiaojing 1; Wang, Yuying ; Tian, Shiping1 ; Qin, Guozheng1
	2021
发表期刊	HORTICULTURE RESEARCH
ISSN	2662-6810
卷号	8 期号:1
摘要	Light plays a critical role in plant growth and development, but the mechanisms through which light regulates fruit ripening and nutritional quality in horticultural crops remain largely unknown. Here, we found that ELONGATED HYPOCOTYL 5 (HY5), a master regulator in the light signaling pathway, is required for normal fruit ripening in tomato (Solanum lycopersicum). Loss of function of tomato HY5 (SlHY5) impairs pigment accumulation and ethylene biosynthesis. Transcriptome profiling identified 2948 differentially expressed genes, which included 1424 downregulated and 1524 upregulated genes, in the Slhy5 mutants. In addition, genes involved in carotenoid and anthocyanin biosynthesis and ethylene signaling were revealed as direct targets of SlHY5 by chromatin immunoprecipitation. Surprisingly, the expression of a large proportion of genes encoding ribosomal proteins was downregulated in the Slhy5 mutants, and this downregulation pattern was accompanied by a decrease in the abundance of ribosomal proteins. Further analysis demonstrated that SlHY5 affected the translation efficiency of numerous ripening-related genes. These data indicate that SlHY5 regulates fruit ripening both at the transcriptional level by targeting specific molecular pathways and at the translational level by affecting the protein translation machinery. Our findings unravel the regulatory mechanisms of SlHY5 in controlling fruit ripening and nutritional quality and uncover the multifaceted regulation of gene expression by transcription factors.
学科领域	Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1038/s41438-021-00523-0
收录类别	SCI
语种	英语
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000636340500020
出版者	NANJING AGRICULTURAL UNIV
文献子类	Article
出版地	NANJING
EISSN	2052-7276
资助机构	National Natural Science Foundation of China [31801601, 31925035, 31871855] ; China Postdoctoral Science Foundation [2018T110153] ; Youth Innovation Promotion Association CAS [2019083]
作者邮箱	gzqin@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：62[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26566
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Weihao,Wang, Peiwen,Li, Xiaojing,et al. The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels[J]. HORTICULTURE RESEARCH,2021,8(1).
APA	Wang, Weihao,Wang, Peiwen,Li, Xiaojing,Wang, Yuying,Tian, Shiping,&Qin, Guozheng.(2021).The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels.HORTICULTURE RESEARCH,8(1).
MLA	Wang, Weihao,et al."The transcription factor SlHY5 regulates the ripening of tomato fruit at both the transcriptional and translational levels".HORTICULTURE RESEARCH 8.1(2021).

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