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| The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress | |
Wang, Tianzuo; Ren, Lifei; Li, Caihong; Zhang, Di; Zhang, Xiuxiu; Zhou, Gang1; Gao, Dan1; Chen, Rujin2; Chen, Yuhui2; Wang, Zhaolan3; Shi, Fengling4; Farmer, Andrew D.; Li, Yansu6; Zhou, Mengyan1; Young, Nevin D.; Zhang, Wen-Hao
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| 2021 | |
| 发表期刊 | BMC BIOLOGY
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| 卷号 | 19期号:1 |
| 摘要 | Background Medicago ruthenica, a wild and perennial legume forage widely distributed in semi-arid grasslands, is distinguished by its outstanding tolerance to environmental stress. It is a close relative of commonly cultivated forage of alfalfa (Medicago sativa). The high tolerance of M. ruthenica to environmental stress makes this species a valuable genetic resource for understanding and improving traits associated with tolerance to harsh environments. Results We sequenced and assembled genome of M. ruthenica using an integrated approach, including PacBio, Illumina, 10xGenomics, and Hi-C. The assembled genome was 904.13 Mb with scaffold N50 of 99.39 Mb, and 50,162 protein-coding genes were annotated. Comparative genomics and transcriptomic analyses were used to elucidate mechanisms underlying its tolerance to environmental stress. The expanded FHY3/FAR1 family was identified to be involved in tolerance of M. ruthenica to drought stress. Many genes involved in tolerance to abiotic stress were retained in M. ruthenica compared to other cultivated Medicago species. Hundreds of candidate genes associated with drought tolerance were identified by analyzing variations in single nucleotide polymorphism using accessions of M. ruthenica with varying tolerance to drought. Transcriptomic data demonstrated the involvements of genes related to transcriptional regulation, stress response, and metabolic regulation in tolerance of M. ruthenica. Conclusions We present a high-quality genome assembly and identification of drought-related genes in the wild species of M. ruthenica, providing a valuable resource for genomic studies on perennial legume forages. |
| 关键词 | Wild genetic resource Genome Evolution Domestication Comparative genomics Transcriptome Single-nucleotide polymorphism Drought tolerance Medicago ruthenica |
| 学科领域 | Biology |
| DOI | 10.1186/s12915-021-01033-0 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | DNA HELICASE 45 ; TRANSCRIPTION FACTORS ; SEQUENCE ; ALIGNMENT ; PROGRAM ; GENES ; RADIATION ; ALFALFA ; LIGHT ; PEA |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000654882900002 |
| 出版者 | BMC |
| 文献子类 | Article |
| 出版地 | LONDON |
| EISSN | 1741-7007 |
| 资助机构 | National Natural Science Foundation of China [32070351, 31830011] ; Chinese Academy of Sciences [KFJ-STSZDTP-056] |
| 作者邮箱 | zhoumengyan@novogene.com ; whzhang@ibcas.ac.cn |
| 作品OA属性 | gold, Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26567 |
| 专题 | 植被与环境变化国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 2.Novogene Bioinformat Inst, Beijing, Peoples R China 3.Lanzhou Univ, Sch Life Sci, Lanzhou, Peoples R China 4.Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China 5.Inner Mongolia Agr Univ, Coll Ecol & Environm Sci, Hohhot, Peoples R China 6.Farmer, Andrew D.] Natl Ctr Genome Resources, Santa Fe, NM USA 7.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing, Peoples R China 8.Young, Nevin D.] Univ Minnesota, Dept Plant Pathol, Minneapolis, MN 55455 USA 9.Young, Nevin D.] Univ Minnesota, Dept Plant Biol, Minneapolis, MN 55455 USA 10.Chinese Acad Sci, Inner Mongolia Res Ctr Prataculture, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Tianzuo,Ren, Lifei,Li, Caihong,et al. The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress[J]. BMC BIOLOGY,2021,19(1). |
| APA | Wang, Tianzuo.,Ren, Lifei.,Li, Caihong.,Zhang, Di.,Zhang, Xiuxiu.,...&Zhang, Wen-Hao.(2021).The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress.BMC BIOLOGY,19(1). |
| MLA | Wang, Tianzuo,et al."The genome of a wild Medicago species provides insights into the tolerant mechanisms of legume forage to environmental stress".BMC BIOLOGY 19.1(2021). |
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| Wang-2021-The genome（3678KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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