Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera

doi:10.3390/molecules26113449

	Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera
	Wang, Fenfen 1; Su, Yalei 1; Chen, Naizhi ; Shen, Shihua
	2021
发表期刊	MOLECULES
卷号	26 期号:11
摘要	Broussonetia papyrifera is a multifunctional deciduous tree that is both a food and a source of traditional Chinese medicine for both humans and animals. Further analysis of the UGT gene family is of great significance to the utilization of B. papyrifera. The substrates of plant UGT genes include highly diverse and complex chemicals, such as flavonoids and terpenes. In order to deepen our understanding of this family, a comprehensive analysis was performed. Phylogenetic analysis showed that 155 BpUGTs were divided into 15 subgroups. A conserved motif analysis showed that BpUGT proteins in the same subgroups possessed similar motif structures. Tandem duplication was the primary driving force for the expansion of the BpUGT gene family. The global promoter analysis indicated that they were associated with complex hormone regulatory networks and the stress response, as well as the synthesis of secondary metabolites. The expression pattern analysis showed that the expression level of BpUGTs in leaves and roots was higher than that in fruits and stems. Next, we determined the composition and content of flavonoids, the main products of the BpUGT reaction. A total of 19 compounds were isolated and analyzed by UPLC-ESI-MS/MS in 3 species of Broussonetia including B. kazinoki, B. papyrifera, and B. kazinoki x B. papyrifera, and the number of compounds was different in these 3 species. The total flavonoid content and antioxidant capacities of the three species were analyzed respectively. All assays exhibited the same trend: the hybrid paper mulberry showed a higher total flavonoid content, a higher total phenol content and higher antioxidant activity than the other two species. Overall, our study provides valuable information for understanding the function of BpUGTs in the biosynthesis of flavonoids.
关键词	Broussonetia polyphenols flavonoid antioxidant activity BpUGTs
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/molecules26113449
收录类别	SCI
语种	英语
WOS关键词	CHLOROGENIC ACID ; IN-VITRO ; GLYCOSYLTRANSFERASES ; PLANT ; BIOSYNTHESIS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000660362000001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1420-3049
资助机构	National Natural Science Foundation of China [31870247]
作者邮箱	wangfenfen@ibcas.ac.cn ; suyalei@ibcas.ac.cn ; chennaizhi@ibcas.ac.cn ; shshen@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26571
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Fenfen,Su, Yalei,Chen, Naizhi,et al. Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera[J]. MOLECULES,2021,26(11).
APA	Wang, Fenfen,Su, Yalei,Chen, Naizhi,&Shen, Shihua.(2021).Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera.MOLECULES,26(11).
MLA	Wang, Fenfen,et al."Genome-Wide Analysis of the UGT Gene Family and Identification of Flavonoids in Broussonetia papyrifera".MOLECULES 26.11(2021).

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