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Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2
Li, Jinjin1; Wang, Weiwei; Yuan, Jinhong1; Xu, Jinyu; He, Lifei; Zhang, Xinying; Zhang, Haiyan
2021
发表期刊PLANT SCIENCE
ISSN0168-9452
卷号304
摘要Plants have evolved sophisticated mechanisms to adjust to deficiency or excess of nutrients. Membrane transport proteins play a central role in nutrient uptake from soil. In Arabidopsis thaliana, the COPPER TRANSPORTOR (COPT) family encodes high-affinity copper transporters. COPT2 is transcriptionally regulated in response to changing levels of cellular copper. However, little is known about whether COPT2 activity is subject to multiple levels of regulation. Here, we showed that the plasma membrane-/endoplasmic reticulum-resident COPT2 protein is degraded in response to excess copper. Confocal microscopy analysis together with pharmacological treatment with a vesicle trafficking inhibitor or vacuolar ATPase inhibitor indicated that copper-mediated downregulation of COPT2 is unlikely to be controlled by endosomal recycling and vacuolar system. However, COPT2 protein is stabilized by proteasome inhibition. Through site-directed mutagenesis, we found that COPT2 cannot be ubiquitinated, and lysine residues at the C-terminus is dispensable for copper-induced degradation of COPT2 but required for copper acquisition. Altogether, our findings reveal that unlike many metal transporters in Arabidopsis, COPT2 is a substrate of ubiquitin-independent proteasomal degradation but not of vacuolar proteases. These findings highlight the mechanistic diversity and complexity of plasma membrane transporter degradation.
关键词COPT2 Arabidopsis Vacuolar degradation Proteasome Ubiquitin Vesicle trafficking
学科领域Biochemistry & Molecular Biology ; Plant Sciences
DOI10.1016/j.plantsci.2021.110825
收录类别SCI
语种英语
WOS关键词PLASMA-MEMBRANE PROTEINS ; ENDOPLASMIC-RETICULUM ; DEFICIENCY RESPONSES ; IRON HOMEOSTASIS ; AUXIN TRANSPORT ; ENDOCYTOSIS ; TRAFFICKING ; EXPRESSION ; ROOT ; ACCUMULATION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000626081800003
出版者ELSEVIER IRELAND LTD
文献子类Article
出版地CLARE
EISSN1873-2259
资助机构National Natural Science Foundation of China [31470292] ; Natural Science Foundation of Tianjin ; [18JCZDJC97900]
作者邮箱skyzhy@tjnu.edu.cn
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26578
专题中科院北方资源植物重点实验室
作者单位1.Tianjin Normal Univ, Coll Life Sci, Tianjin Key Lab Anim & Plant Resistance, Tianjin 300387, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
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GB/T 7714
Li, Jinjin,Wang, Weiwei,Yuan, Jinhong,et al. Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2[J]. PLANT SCIENCE,2021,304.
APA Li, Jinjin.,Wang, Weiwei.,Yuan, Jinhong.,Xu, Jinyu.,He, Lifei.,...&Zhang, Haiyan.(2021).Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2.PLANT SCIENCE,304.
MLA Li, Jinjin,et al."Ubiquitin-independent proteasome system is required for degradation of Arabidopsis COPPER TRANSPORTER 2".PLANT SCIENCE 304(2021).
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