The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen

doi:10.1080/15592324.2021.1924502

	The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen
	Wang, Yanli 1; Wang, Tai1,2 ; Liu, Lingtong
	2021
发表期刊	PLANT SIGNALING & BEHAVIOR
ISSN	1559-2316
卷号	16 期号:8
摘要	Cell-type-specific markers are valuable tools to reveal developmental processes underlying cell differentiation during plant reproduction. Here we report the pollen vegetative cell marker gene VCS1 (Vegetative Cell Specific 1) of rice (Oryza sativa japonica). VCS1 was expressed specifically in late pollen and was predicted to encode a small FAF domain-containing protein of 205 amino acid residues (aa). The expression of reporter fusion proteins showed that VCS1 was exclusively targeted to the vegetative nucleus of pollen. Upon pollen germination, VCS1 lost vegetative nucleus localization, and appeared diffused in the vegetative cytoplasm of pollen grain but not in the pollen tube. T-DNA insertional mutation which disrupted the carboxyl-terminal 21 aa of VCS1 did not affect plant vegetative growth and pollen development, while destruction of VCS1 by CRISPR/Cas9 only moderately affect pollen viability. VCS1 is evolutionally conserved in monocots but appeared absent in dicotyledons. This study reveals a molecular tool for visualizing the vegetative cell of rice and possible other monocots, which has potential values in the genetic engineering of male-sterile lines.
关键词	Pollen rice vegetative nucleus DUF3049 FANTASTIC FOUR
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1080/15592324.2021.1924502
收录类别	SCI
语种	英语
WOS关键词	DNA INSERTIONAL MUTAGENESIS ; GENE-EXPRESSION ; SPERM CELLS ; ZEA-MAYS ; PROMOTERS ; VECTORS ; PROTEIN ; SYSTEM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000650561000001
出版者	TAYLOR & FRANCIS INC
文献子类	Article
出版地	PHILADELPHIA
EISSN	1559-2324
资助机构	National Natural Science Foundation of China [32000247] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
作者邮箱	twang@ibcas.ac.cnis ; ltliu@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26581
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanli,Wang, Tai,Liu, Lingtong. The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen[J]. PLANT SIGNALING & BEHAVIOR,2021,16(8).
APA	Wang, Yanli,Wang, Tai,&Liu, Lingtong.(2021).The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen.PLANT SIGNALING & BEHAVIOR,16(8).
MLA	Wang, Yanli,et al."The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen".PLANT SIGNALING & BEHAVIOR 16.8(2021).

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