Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy

doi:10.3390/membranes11100747

	Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy
	Tang, Ling 1; Li, Yang 1; Zhong, Cheng 2; Deng, Xin; Wang, Xiaohua
	2021
发表期刊	MEMBRANES
卷号	11 期号:10
摘要	Local inhomogeneities in lipid composition play a crucial role in the regulation of signal transduction and membrane traffic. This is particularly the case for plant plasma membrane, which is enriched in specific lipids, such as free and conjugated forms of phytosterols and typical phytosphingolipids. Nevertheless, most evidence for microdomains in cells remains indirect, and the nature of membrane inhomogeneities has been difficult to characterize. We used a new push-pull pyrene probe and fluorescence lifetime imaging microscopy (FLIM) combined with all-atom multiscale molecular dynamics simulations to provide a detailed view on the interaction between phospholipids and phytosterol and the effect of modulating cellular phytosterols on membrane-associated microdomains and phase separation formation. Our understanding of the organization principles of biomembranes is limited mainly by the challenge to measure distributions and interactions of lipids and proteins within the complex environment of living cells. Comparing phospholipids/phytosterol compositions typical of liquid-disordered (Ld) and liquid-ordered (Lo) domains, we furthermore show that phytosterols play crucial roles in membrane homeostasis. The simulation work highlights how state-of-the-art modeling alleviates some of the prior concerns and how unrefuted discoveries can be made through a computational microscope. Altogether, our results support the role of phytosterols in the lateral structuring of the PM of plant cells and suggest that they are key compounds for the formation of plant PM microdomains and the lipid-ordered phase.
关键词	FLIM computational microscopy phase separation microdomain phytosterol
学科领域	Biochemistry & Molecular Biology ; Chemistry, Physical ; Engineering, Chemical ; Materials Science, Multidisciplinary ; Polymer Science
DOI	10.3390/membranes11100747
收录类别	SCI
语种	英语
WOS关键词	X-RAY-DIFFRACTION ; MOLECULAR-DYNAMICS SIMULATIONS ; BREWSTER-ANGLE MICROSCOPY ; PLASMA-MEMBRANE ; LIPID ORDER ; FREE-ENERGY ; ARABIDOPSIS ; CHOLESTEROL ; PHOSPHOLIPIDS ; VISUALIZATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000717013600001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	2077-0375
资助机构	National Natural Science Foundation of China [3217020622, 31770400, 31771493]
作者邮箱	tangling181@mails.ucas.edu.cn ; liyang184@mails.ucas.edu.cn ; zhongcheng@whu.edu.cn ; deng@ibcas.ac.cn ; wangxh@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26588
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 3.Wuhan Univ, Dept Chem, Hubei Key Lab Organ & Polymer Optoelect Mat, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Tang, Ling,Li, Yang,Zhong, Cheng,et al. Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy[J]. MEMBRANES,2021,11(10).
APA	Tang, Ling,Li, Yang,Zhong, Cheng,Deng, Xin,&Wang, Xiaohua.(2021).Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy.MEMBRANES,11(10).
MLA	Tang, Ling,et al."Plant Sterol Clustering Correlates with Membrane Microdomains as Revealed by Optical and Computational Microscopy".MEMBRANES 11.10(2021).

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