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Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily
You, Chen1; Zhang, YuPing; Yang, ShaoYu; Wang, Xu; Yao, Wen; Jin, WeiHuan; Wang, Wei; Hu, XiuLi; Yang, Hao2
2021
发表期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
卷号12
摘要In plants, the cell fates of a vegetative cell (VC) and generative cell (GC) are determined after the asymmetric division of the haploid microspore. The VC exits the cell cycle and grows a pollen tube, while the GC undergoes further mitosis to produce two sperm cells for double fertilization. However, our understanding of the mechanisms underlying their fate differentiation remains limited. One major advantage of the nuclear proteome analysis is that it is the only method currently able to uncover the systemic differences between VC and GC due to GC being engulfed within the cytoplasm of VC, limiting the use of transcriptome. Here, we obtained pure preparations of the vegetative cell nuclei (VNs) and generative cell nuclei (GNs) from germinating lily pollens. Utilizing these high-purity VNs and GNs, we compared the differential nucleoproteins between them using state-of-the-art quantitative proteomic techniques. We identified 720 different amount proteins (DAPs) and grouped the results in 11 fate differentiation categories. Among them, we identified 29 transcription factors (TFs) and 10 cell fate determinants. Significant differences were found in the molecular activities of vegetative and reproductive nuclei. The TFs in VN mainly participate in pollen tube development. In comparison, the TFs in GN are mainly involved in cell differentiation and male gametogenesis. The identified novel TFs may play an important role in cell fate differentiation. Our data also indicate differences in nuclear pore complexes and epigenetic modifications: more nucleoporins synthesized in VN; more histone variants and chaperones; and structural maintenance of chromosome (SMC) proteins, chromatin remodelers, and DNA methylation-related proteins expressed in GN. The VC has active macromolecular metabolism and mRNA processing, while GC has active nucleic acid metabolism and translation. Moreover, the members of unfolded protein response (UPR) and programmed cell death accumulate in VN, and DNA damage repair is active in GN. Differences in the stress response of DAPs in VN vs. GN were also found. This study provides a further understanding of pollen cell differentiation mechanisms and also a sound basis for future studies of the molecular mechanisms behind cell fate differentiation.
关键词cell differentiation pollen vegetative cell generative cell nuclear proteins lily proteomic
学科领域Plant Sciences
DOI10.3389/fpls.2021.641517
收录类别SCI
语种英语
WOS关键词DNA-BINDING PROTEIN ; MALE GAMETIC CELLS ; TUBE GROWTH ; ARABIDOPSIS-THALIANA ; HISTONE CHAPERONE ; GAMETOPHYTE DEVELOPMENT ; MALE GAMETOGENESIS ; PORE COMPLEX ; TRANSCRIPTION ; GENE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000663825500001
出版者FRONTIERS MEDIA SA
文献子类Article
出版地LAUSANNE
资助机构Youth Program of National Natural Science Foundation of China [31800260] ; Key Laboratory of Plant Molecular Physiology, Chinese Academy of Sciences fund
作者邮箱dna_gtp@163.com
作品OA属性Green Published, gold
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26611
专题中科院植物分子生理学重点实验室
作者单位1.Henan Agr Univ, Coll Life Sci, State Key Lab Wheat & Maize Crop Sci, Zhengzhou, Peoples R China
2.Henan Normal Univ, Coll Life Sci, Xinxiang, Henan, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
推荐引用方式
GB/T 7714
You, Chen,Zhang, YuPing,Yang, ShaoYu,et al. Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily[J]. FRONTIERS IN PLANT SCIENCE,2021,12.
APA You, Chen.,Zhang, YuPing.,Yang, ShaoYu.,Wang, Xu.,Yao, Wen.,...&Yang, Hao.(2021).Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily.FRONTIERS IN PLANT SCIENCE,12.
MLA You, Chen,et al."Proteomic Analysis of Generative and Vegetative Nuclei Reveals Molecular Characteristics of Pollen Cell Differentiation in Lily".FRONTIERS IN PLANT SCIENCE 12(2021).
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