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| A Small Guanosine Triphosphate Binding Protein PagRabE1b Promotes Xylem Development in Poplar | |
| Liu, Ying-Li; Wang, Li-Juan; Li, Yu2; Guo, Ying-Hua; Cao, Yuan; Zhao, Shu-Tang | |
| 2021 | |
| Source Publication | FRONTIERS IN PLANT SCIENCE
 |
| ISSN | 1664-462X |
| Volume | 12 |
| Abstract | Rab GTPases are the subfamily of the small guanosine triphosphate (GTP)-binding proteins which participated in the regulation of various biological processes. Recent studies have found that plant Rabs play some specific functions. However, the functions of Rabs in xylem development in trees remain unclear. In this study, functional identification of PagRabE1b in Populus was performed. Quantitative reverse transcription PCR (qRT-PCR) results showed that PagRabE1b was highly accumulated in stems, especially in phloem and xylem tissues. Overexpression of PagRabE1b in poplar enhanced programmed cell death (PCD) and increased the growth rate and the secondary cell wall (SCW) thickness. Quantitative analysis of monosaccharide content showed that various monosaccharides were significantly increased in secondary xylem tissues of the overexpressed lines. Flow cytometry analysis revealed that the number of apoptotic cells in PagRabE1b-OE lines is more than a wild type (WT), which indicated that PagRabE1b may play an important role in PCD. Further studies showed that overexpression of PagRabE1b increased the expression level of genes involved in SCW biosynthesis, PCD, and autophagy. Collectively, the results suggest that PagRabE1b plays a positive role in promoting the xylem development of poplar. |
| Keyword | poplar small gtp binding protein PagRabE1b cell wall wood formation |
| Subject Area | Plant Sciences |
| DOI | 10.3389/fpls.2021.686024 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | CELL-WALL BIOSYNTHESIS ; WOOD FORMATION ; FAMILY GENES ; SMALL GTPASE ; ARABIDOPSIS ; RHO ; PATHWAY ; GROWTH ; DEATH ; SND1 |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000662945800001 |
| Publisher | FRONTIERS MEDIA SA |
| Subtype | Article |
| Publication Place | LAUSANNE |
| Funding Organization | National Nonprofit Institute Research Grant of CAF [CAFYBB2012039] ; National Key Program on Transgenic Research [2018ZX08020002] |
| Corresponding Author Email | zhaost318@163.com |
| OA | gold, Green Published |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/26612 |
| Collection | 中科院植物分子生理学重点实验室 |
| Affiliation | 1.Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China 2.Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China |
| Recommended Citation GB/T 7714 | Liu, Ying-Li,Wang, Li-Juan,Li, Yu,et al. A Small Guanosine Triphosphate Binding Protein PagRabE1b Promotes Xylem Development in Poplar[J]. FRONTIERS IN PLANT SCIENCE,2021,12. |
| APA | Liu, Ying-Li,Wang, Li-Juan,Li, Yu,Guo, Ying-Hua,Cao, Yuan,&Zhao, Shu-Tang.(2021).A Small Guanosine Triphosphate Binding Protein PagRabE1b Promotes Xylem Development in Poplar.FRONTIERS IN PLANT SCIENCE,12. |
| MLA | Liu, Ying-Li,et al."A Small Guanosine Triphosphate Binding Protein PagRabE1b Promotes Xylem Development in Poplar".FRONTIERS IN PLANT SCIENCE 12(2021). |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| S1664462X_S1664462X2（2862KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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