Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe

doi:10.1016/j.apsoil.2021.104054

	Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe
	Niu, Guoxiang 1; Hasi, Muqier 1; Wang, Ruzhen 2; Wang, Yinliu 1; Geng, Qianqian 1; Hu, Shuya 1; Xu, Xiaohui 1; Yang, Junjie 1; Wang, Changhui ; Han, Xingguo1 ; Huang, Jianhui1
	2021
发表期刊	APPLIED SOIL ECOLOGY
ISSN	0929-1393
卷号	167
摘要	Global nitrogen (N) deposition has been influencing the structure of soil microbial communities, which play fundamental roles in modifying most biogeochemical processes. However, to date, our understanding of how long-term N deposition affects soil microbial communities, particularly those in subsurface soil, is largely incomplete. In this study, we examined soil microbial phospholipid fatty acids at different depths in the soil profile, including in topsoil (0-10 cm), midsoil (30-40 cm), and subsoil (70-100 cm), in a 10-year N addition experimental site in a semiarid steppe. We collected soil samples at four N addition treatment levels, including 0, 2, 10, and 50 g M-2 year(-1), which represented control, low-N, medium-N, and high-N inputs, respectively. Our results showed that N addition remarkably shifted soil microbial communities by increasing the relative abundances of bacteria and Gram-positive (GP) bacteria, and decreasing Gram-negative (GN) bacterial relative abundance across the three soil layers by affecting soil pH and NO3-N, particularly under the medium- and highN addition rates. Moreover, the effects of N addition tended to increase with the N addition rate but diminished with soil depth. Soil pH, NO3-N, and NH4-N were the most important driving factors for changes in microbial community composition in topsoil, whereas soil total N (TN) and total carbon were important in midsoil, and TN, dissolved organic N, and soil moisture were important in subsoil. The relative abundances of bacteria and GN bacteria also decreased, whereas those of fungi and GP bacteria increased with soil depth regardless of N addition. Soil TN and pH were the most important factors in shaping the vertical distribution of soil microbial communities. Our results suggest that both N addition and soil depth may cause similar microbial community shifts, through which fungi and GP bacteria become dominant, but through different mechanisms. These results suggest that it is important to distinguish among the N-deposition effects on soil microbial communities at different soil depths when building soil system models.
关键词	Mongolian Plateau N deposition Soil microbiome Steppe Soil profile Vertical distribution
学科领域	Soil Science
DOI	10.1016/j.apsoil.2021.104054
收录类别	SCI
语种	英语
WOS关键词	CARBON-USE EFFICIENCY ; ORGANIC-CARBON ; PATTERNS ; DEPOSITION ; BACTERIAL ; BIOMASS ; BIODIVERSITY ; MYCORRHIZAL ; SUBSOIL ; FUNGAL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000691498900005
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1873-0272
资助机构	Inner Mongolia Grassland Ecosystem Research Station ; National Natural Science Foundation of China [31870440, 32071562]
作者邮箱	jhhuang@ibcas.ac.cn
引用统计	被引频次：27[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26617
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Appl Ecol, Erguna Forest Steppe Ecotone Ecosyst Res Stn, Shenyang 110016, Peoples R China
推荐引用方式 GB/T 7714	Niu, Guoxiang,Hasi, Muqier,Wang, Ruzhen,et al. Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe[J]. APPLIED SOIL ECOLOGY,2021,167.
APA	Niu, Guoxiang.,Hasi, Muqier.,Wang, Ruzhen.,Wang, Yinliu.,Geng, Qianqian.,...&Huang, Jianhui.(2021).Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe.APPLIED SOIL ECOLOGY,167.
MLA	Niu, Guoxiang,et al."Soil microbial community responses to long-term nitrogen addition at different soil depths in a typical steppe".APPLIED SOIL ECOLOGY 167(2021).

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