Knowledge Management System Of Institute Of Botany,CAS
Determination of a criminal suspect using environmental plant DNA metabarcoding technology | |
Liu, Yanlei1; Xu, Chao; Dong, Wenpan2; Yang, Xueying3; Zhou, Shiliang | |
2021 | |
发表期刊 | FORENSIC SCIENCE INTERNATIONAL |
ISSN | 0379-0738 |
卷号 | 324 |
摘要 | There are criminal cases that no frequently used evidence, for example, human DNAs from the criminal, is available. Such cases usually are unresolvable. With the advent of DNA metabarcoding, evidences are mined from environmental DNA and such cases become resolvable. This study reports how a criminal suspect was determined by environmental plant DNA metabarcoding technology. A girl was killed in a rural wet area in China without a witness or video record. Pants with dried mud was found from one of her classmate's house. The mud was removed from the pants and 11 more mud or soil samples surrounding murder scene were collected. DNA was extracted from the soil. Chloroplast rbcL gene were amplified and sequenced on a next generation sequencing platform. After bioinformatics analysis, ZOTU composition of 12 samples de-monstrated that the mud on the suspect's pants was from the criminal scene. The suspect finally made a clean breast of his crime. This case implies that plant DNA in the environment soil is a new source of evidence in determination of suspects using DNA metabarcoding technology and has high potentials of extensive applications in criminal cases. (C) 2021 Elsevier B.V. All rights reserved. |
关键词 | DNA barcoding Forensics DNA metabarcoding Environmental plant DNA |
学科领域 | Medicine, Legal |
DOI | 10.1016/j.forsciint.2021.110828 |
收录类别 | SCI |
语种 | 英语 |
WOS关键词 | BARCODE |
WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
WOS记录号 | WOS:000755969300012 |
出版者 | ELSEVIER IRELAND LTD |
文献子类 | Article |
出版地 | CLARE |
EISSN | 1872-6283 |
资助机构 | National Key R&D Program of China [2017YFC0803803] ; Open Project of Institute of Forensic Science, Ministry of Public Security [2018FGKFKT04] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA23080204, XDA19050303] ; National Natural Science Foundation of China [NSFC31872679] ; fundamental research fund of the Central Public Service Research Institute [2018JB001] |
作者邮箱 | yxystyhhp@163.com ; slzhou@ibcas.ac.cn |
作品OA属性 | Green Submitted |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26619 |
专题 | 系统与进化植物学国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Beijing Forestry Univ, Sch Ecol & Nat Conservat, Lab Systemat Evolut & Biogeog Woody Plants, Beijing 100083, Peoples R China 4.Minist Publ Secur, Inst Forens Sci, Key Lab Forens Genet, Natl Engn Lab Forens Sci, Beijing 100038, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, Yanlei,Xu, Chao,Dong, Wenpan,et al. Determination of a criminal suspect using environmental plant DNA metabarcoding technology[J]. FORENSIC SCIENCE INTERNATIONAL,2021,324. |
APA | Liu, Yanlei,Xu, Chao,Dong, Wenpan,Yang, Xueying,&Zhou, Shiliang.(2021).Determination of a criminal suspect using environmental plant DNA metabarcoding technology.FORENSIC SCIENCE INTERNATIONAL,324. |
MLA | Liu, Yanlei,et al."Determination of a criminal suspect using environmental plant DNA metabarcoding technology".FORENSIC SCIENCE INTERNATIONAL 324(2021). |
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