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Determination of a criminal suspect using environmental plant DNA metabarcoding technology
Liu, Yanlei1; Xu, Chao; Dong, Wenpan2; Yang, Xueying3; Zhou, Shiliang
2021
发表期刊FORENSIC SCIENCE INTERNATIONAL
ISSN0379-0738
卷号324
摘要There are criminal cases that no frequently used evidence, for example, human DNAs from the criminal, is available. Such cases usually are unresolvable. With the advent of DNA metabarcoding, evidences are mined from environmental DNA and such cases become resolvable. This study reports how a criminal suspect was determined by environmental plant DNA metabarcoding technology. A girl was killed in a rural wet area in China without a witness or video record. Pants with dried mud was found from one of her classmate's house. The mud was removed from the pants and 11 more mud or soil samples surrounding murder scene were collected. DNA was extracted from the soil. Chloroplast rbcL gene were amplified and sequenced on a next generation sequencing platform. After bioinformatics analysis, ZOTU composition of 12 samples de-monstrated that the mud on the suspect's pants was from the criminal scene. The suspect finally made a clean breast of his crime. This case implies that plant DNA in the environment soil is a new source of evidence in determination of suspects using DNA metabarcoding technology and has high potentials of extensive applications in criminal cases. (C) 2021 Elsevier B.V. All rights reserved.
关键词DNA barcoding Forensics DNA metabarcoding Environmental plant DNA
学科领域Medicine, Legal
DOI10.1016/j.forsciint.2021.110828
收录类别SCI
语种英语
WOS关键词BARCODE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000755969300012
出版者ELSEVIER IRELAND LTD
文献子类Article
出版地CLARE
EISSN1872-6283
资助机构National Key R&D Program of China [2017YFC0803803] ; Open Project of Institute of Forensic Science, Ministry of Public Security [2018FGKFKT04] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA23080204, XDA19050303] ; National Natural Science Foundation of China [NSFC31872679] ; fundamental research fund of the Central Public Service Research Institute [2018JB001]
作者邮箱yxystyhhp@163.com ; slzhou@ibcas.ac.cn
作品OA属性Green Submitted
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26619
专题系统与进化植物学国家重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Beijing Forestry Univ, Sch Ecol & Nat Conservat, Lab Systemat Evolut & Biogeog Woody Plants, Beijing 100083, Peoples R China
4.Minist Publ Secur, Inst Forens Sci, Key Lab Forens Genet, Natl Engn Lab Forens Sci, Beijing 100038, Peoples R China
推荐引用方式
GB/T 7714
Liu, Yanlei,Xu, Chao,Dong, Wenpan,et al. Determination of a criminal suspect using environmental plant DNA metabarcoding technology[J]. FORENSIC SCIENCE INTERNATIONAL,2021,324.
APA Liu, Yanlei,Xu, Chao,Dong, Wenpan,Yang, Xueying,&Zhou, Shiliang.(2021).Determination of a criminal suspect using environmental plant DNA metabarcoding technology.FORENSIC SCIENCE INTERNATIONAL,324.
MLA Liu, Yanlei,et al."Determination of a criminal suspect using environmental plant DNA metabarcoding technology".FORENSIC SCIENCE INTERNATIONAL 324(2021).
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