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Redox proteomic analysis reveals the involvement of oxidative post-translational modification in tomato fruit ripening
Wang, Peiwen1; Li, Xiaojing1; Wang, Yuying; Wang, Weihao; Tian, Shiping1; Qin, Guozheng1
2021
发表期刊POSTHARVEST BIOLOGY AND TECHNOLOGY
ISSN0925-5214
卷号178
摘要Fruit ripening is a complicated biological process, in which the levels of reactive oxygen species (ROS) fluctuate. However, the molecular mechanisms of ROS in the regulation of fruit ripening are still unclear. Here we utilized an iodoacetyl tandem mass tag (iodoTMT)-based redox proteomic approach to detect the redox dynamics of proteins during tomato fruit ripening. A total of 70 cysteine (Cys)-containing peptides from 51 proteins were identified in fruit at different ripening stages. Determination of oxidation levels indicated that Cys-containing peptides at the later stage of fruit ripening were generally more oxidized than those at the early stage. Of these proteins, 13 were identified as redox-sensitive proteins, whose oxidation levels changed significantly during fruit ripening, including polygalacturonase 2A (PG2A) involved in cell wall degradation and 1-aminocyclopropane-1-carboxylate oxidase-like protein (E8) involved in ethylene biosynthesis. Immunodetection of oxidized Cys residues demonstrated that Cys-307 in E8 was a redox-sensitive site, whose oxidation is likely to affect the function of E8. These data suggest that ROS may regulate fruit ripening by targeting critical enzymes via oxidative post-translational modifications. Our findings uncover the mechanisms of ROS in the regulation of fruit ripening and contribute to the regulatory networks controlling ripening.
关键词Tomato Fruit ripening Reactive oxygen species iodoTMT Redox proteomics Oxidative modification
学科领域Agronomy ; Food Science & Technology ; Horticulture
DOI10.1016/j.postharvbio.2021.111556
收录类别SCI
语种英语
WOS关键词ETHYLENE ; EXPRESSION ; DISULFIDE ; STRESS ; POLYGALACTURONASE ; IDENTIFICATION ; BIOSYNTHESIS ; LYCOPERSICON ; GENERATION ; SENESCENCE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000646238000005
出版者ELSEVIER
文献子类Article
出版地AMSTERDAM
EISSN1873-2356
资助机构National Natural Science Foundation of China [31925035, 31871855, 32072636]
作者邮箱gzqin@ibcas.ac.cn
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26652
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, 20 Nanxincun, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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GB/T 7714
Wang, Peiwen,Li, Xiaojing,Wang, Yuying,et al. Redox proteomic analysis reveals the involvement of oxidative post-translational modification in tomato fruit ripening[J]. POSTHARVEST BIOLOGY AND TECHNOLOGY,2021,178.
APA Wang, Peiwen,Li, Xiaojing,Wang, Yuying,Wang, Weihao,Tian, Shiping,&Qin, Guozheng.(2021).Redox proteomic analysis reveals the involvement of oxidative post-translational modification in tomato fruit ripening.POSTHARVEST BIOLOGY AND TECHNOLOGY,178.
MLA Wang, Peiwen,et al."Redox proteomic analysis reveals the involvement of oxidative post-translational modification in tomato fruit ripening".POSTHARVEST BIOLOGY AND TECHNOLOGY 178(2021).
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