Gene expression analysis in leaf of Camellia sinensis reveals the response to fluoride

doi:10.1007/s11738-021-03283-5

IB-CAS > 中科院光生物学重点实验室

	Gene expression analysis in leaf of Camellia sinensis reveals the response to fluoride
	Pan, Junting 1; Xing, Anqi ; Zhu, Jiaojiao ; Nong, Shouhua ; Ma, Yuanchun ; Zhu, Xujun ; Fang, Wanping ; Wang, Yuhua
	2021
发表期刊	ACTA PHYSIOLOGIAE PLANTARUM
ISSN	0137-5881
卷号	43 期号:7
摘要	Tea plants (Camellia sinensis (L.) Kuntze) can hyperaccumulate fluoride (F-) without any toxic symptom, especially in mature leaves. However, the molecular mechanism of absorption and hyperaccumulation of F- was poorly understood in C. sinensis. Here, a transcriptomic analysis was performed to compare the responses to fluoride stress of tea leaves to understand F- accumulation and its influence on gene expression in C. sinensis leaves. The results indicated that numerous differentially expressed genes involved in ion absorption and transport exist in the process of F- absorption in C. sinensis leaves. The up-regulated expression of genes associated with uptake and transport of Ca2+ and K+ (CNGC, TPC1, CAX, and VHA) increased the intracellular and vacuole cation concentration during F- accumulation in C. sinensis leaves. We also found that some of AQP (PIPs and TIPs) genes may promote F- into the intracellular spaces and vacuoles in C. sinensis leaves, respectively. In addition, F- induced the expression of differential genes related to plant hormone metabolism and signaling pathways (ABA, auxin, and GA). Subsequently, a lot of transcription factors (WRKY, MYB, NAC, bHLH and AP2/ERF) were activated to respond to F- stress in tea leaves by regulating plant hormone signal transduction and other pathways. And ubiquitin systems were involved in the regulation of proteins homeostasis under F- stress indicating that they are responsible for the response of C. sinensis leaves to F-. Overall, these findings provide a comprehensive understanding of the F- absorption mechanism and its influence on gene expression in tea leaves.
关键词	Camellia sinensis Fluoride Gene expression analysis RNA-Seq
学科领域	Plant Sciences
DOI	10.1007/s11738-021-03283-5
收录类别	SCI
语种	英语
WOS关键词	ABSCISIC-ACID ; TRANSCRIPTION FACTOR ; RNA-SEQ ; TEA ; CALCIUM ; SALT ; ARABIDOPSIS ; TOLERANCE ; TOXICITY ; ALUMINUM
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000668889200001
出版者	SPRINGER HEIDELBERG
文献子类	Article
出版地	HEIDELBERG
EISSN	1861-1664
资助机构	National Natural Science Foundation of China [31972458] ; earmarked fund for Jiangsu Agricultural Industry Technology System [JATS[2020]405] ; earmarked fund for China Agriculture Research System [CARS-19]
作者邮箱	panjunting@ibeas.ac.cn ; 2017104087@njau.edu.cn ; 2016104092@njau.edu.cn ; 2017804197@njau.edu.cn ; myc@njau.edu.cn ; zhuxujun@njau.edu.cn ; fangwp@njau.edu.cn ; wangyuhua@njau.edu.cn
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26663
专题	中科院光生物学重点实验室
作者单位	1.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Pan, Junting,Xing, Anqi,Zhu, Jiaojiao,et al. Gene expression analysis in leaf of Camellia sinensis reveals the response to fluoride[J]. ACTA PHYSIOLOGIAE PLANTARUM,2021,43(7).
APA	Pan, Junting.,Xing, Anqi.,Zhu, Jiaojiao.,Nong, Shouhua.,Ma, Yuanchun.,...&Wang, Yuhua.(2021).Gene expression analysis in leaf of Camellia sinensis reveals the response to fluoride.ACTA PHYSIOLOGIAE PLANTARUM,43(7).
MLA	Pan, Junting,et al."Gene expression analysis in leaf of Camellia sinensis reveals the response to fluoride".ACTA PHYSIOLOGIAE PLANTARUM 43.7(2021).

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