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| Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways | |
| Mitra, Ankita; Ahuja, Akash; Rahmawati, Laily; Kim, Han Gyung; Woo, Byoung Young1; Hong, Yong Deog1; Hossain, Mohammad Amjad; Zhang, Zhiyun3; Kim, Soo-Yong; Lee, Jongsung; Kim, Jong-Hoon; Cho, Jae Youl | |
| 2021 | |
| 发表期刊 | MOLECULES
 |
| 卷号 | 26期号:21 |
| 摘要 | Caragana rosea Turcz, which belongs to the Leguminosae family, is a small shrub found in Northern and Eastern China that is known to possess anti-inflammatory properties and is used to treat fever, asthma, and cough. However, the underlying molecular mechanisms of its anti-inflammatory effects are unknown. Therefore, we used lipopolysaccharide (LPS) in RAW264.7 macrophages to investigate the molecular mechanisms that underlie the anti-inflammatory activities of a methanol extract of Caragana rosea (Cr-ME). We showed that Cr-ME reduced the production of nitric oxide (NO) and mRNA levels of iNOS, TNF-alpha, and IL-6 in a concentration-dependent manner. We also found that Cr-ME blocked MyD88- and TBK1-induced NF-kappa B and IRF3 promoter activity, suggesting that it affects multiple targets. Moreover, Cr-ME reduced the phosphorylation levels of I kappa B alpha, IKK alpha/beta and IRF3 in a time-dependent manner and regulated the upstream NF-kappa B proteins Syk and Src, and the IRF3 protein TBK1. Upon overexpression of Src and TBK1, Cr-ME stimulation attenuated the phosphorylation of the NF-kappa B subunits p50 and p65 and IRF3 signaling. Together, our results suggest that the anti-inflammatory activity of Cr-ME occurs by inhibiting the NF-kappa B and IRF3 signaling pathways. |
| 关键词 | Caragana rosea Turcz (Cr-ME) anti-inflammatory activity NF-kappa B and IRF3 signaling pathways |
| 学科领域 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| DOI | 10.3390/molecules26216660 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | TOLL-LIKE RECEPTORS ; KAPPA-B ; TYROSINE KINASE ; IN-VITRO ; RECOGNITION ; EXPRESSION ; CELLS ; ASSAY ; IRF3 ; L. |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS记录号 | WOS:000719453400001 |
| 出版者 | MDPI |
| 文献子类 | Article |
| 出版地 | BASEL |
| EISSN | 1420-3049 |
| 资助机构 | Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2017R1A6A1A03015642] ; National Institute of Biological Resources ; Ministry of Environment [NIBR202006202] ; Republic of Korea |
| 作者邮箱 | ankitamitra@skku.edu ; akashahuja1988@gmail.com ; lyrahma0106@g.skku.edu ; hanks523@skku.edu ; quddud@amorepacific.com ; hydhong@amorepacific.com ; mamjadh2@gmail.com ; zhangzy@ibcas.ac.cn ; soodole@kribb.re.kr ; bioneer@skku.edu ; jhkim1@chonbuk.ac.kr ; jaecho@skku.edu |
| 作品OA属性 | gold, Green Published |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/26670 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Sungkyunkwan Univ, Dept Integrat Biotechnol, Suwon 16419, South Korea 2.AmorePacif R&D Ctr, Yongin 17074, South Korea 3.Chonbuk Natl Univ, Coll Med, Dept Vet Physiol, Iksan 54596, South Korea 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea |
| 推荐引用方式 GB/T 7714 | Mitra, Ankita,Ahuja, Akash,Rahmawati, Laily,et al. Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways[J]. MOLECULES,2021,26(21). |
| APA | Mitra, Ankita.,Ahuja, Akash.,Rahmawati, Laily.,Kim, Han Gyung.,Woo, Byoung Young.,...&Cho, Jae Youl.(2021).Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways.MOLECULES,26(21). |
| MLA | Mitra, Ankita,et al."Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways".MOLECULES 26.21(2021). |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Mitra-2021-Caragana （4399KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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