Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination

doi:10.1038/s41598-021-90270-y

	Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination
	Mei, Mei ; Wei, Jun 1; Ai, Wanfeng ; Zhang, Lijie ; Lu, Xiu-jun
	2021
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	11 期号:1
摘要	Magnolia sieboldii K. Koch (M. sieboldii) is a deciduous Chinese tree species of the Magnoliaceae family with high ornamental, medicinal, and economic benefits. The germination of M. sieboldii seeds under natural conditions is extremely difficult, thereby hindering the cultivation and breeding of this important species. The molecular mechanisms underlying M. sieboldii seed germination remain unclear due to the lack of genomic and transcriptomic resources. Here, we integrated both mRNA and miRNA sequencing to identify the genes and pathways related to M. sieboldii germination. A comprehensive full-length transcriptome containing 158,083 high-quality unigenes was obtained by single-molecule real-time (SMRT) sequencing technology. We identified a total of 13,877 genes that were differentially expressed between non-germinated and germinated seeds. These genes were mainly involved in plant hormone signal transduction and diverse metabolic pathways such as those involving lipids, sugars, and amino acids. Our results also identified a complex regulatory network between miRNAs and their target genes. Taken together, we present the first transcriptome of M. sieboldii and provide key genes and pathways associated with seed germination for further characterization. Future studies of the molecular basis of seed germination will facilitate the genetic improvement M. sieboldii.
学科领域	Multidisciplinary Sciences
DOI	10.1038/s41598-021-90270-y
收录类别	SCI
语种	英语
WOS关键词	DORMANCY RELEASE ; TRANSCRIPTOME ; GENES ; ARABIDOPSIS ; CYTOCHROME-P450 ; METABOLISM ; MICRORNAS ; PROTEINS ; GROWTH ; TIME
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000659129600012
出版者	NATURE RESEARCH
文献子类	Article
出版地	BERLIN
资助机构	National Natural Science Foundation of China [31570621, 31971647]
作者邮箱	lxjsyau@syau.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26673
专题	中科院植物分子生理学重点实验室
作者单位	1.Shenyang Agr Univ, Dept Hort, Shenyang, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 3.Shenyang Agr Univ, Dept Forestry, Shenyang, Peoples R China
推荐引用方式 GB/T 7714	Mei, Mei,Wei, Jun,Ai, Wanfeng,et al. Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination[J]. SCIENTIFIC REPORTS,2021,11(1).
APA	Mei, Mei,Wei, Jun,Ai, Wanfeng,Zhang, Lijie,&Lu, Xiu-jun.(2021).Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination.SCIENTIFIC REPORTS,11(1).
MLA	Mei, Mei,et al."Integrated RNA and miRNA sequencing analysis reveals a complex regulatory network of Magnolia sieboldii seed germination".SCIENTIFIC REPORTS 11.1(2021).

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