Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza

doi:10.1038/s41467-021-20959-1

	Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza
	Ma, Ying ; Cui, Guanghong ; Chen, Tong ; Ma, Xiaohui 1; Wang, Ruishan ; Jin, Baolong ; Yang, Jian ; Kang, Liping ; Tang, Jinfu ; Lai, Changjiangsheng ; Wang, Yanan ; Zhao, Yujun ; Shen, Ye ; Zeng, Wen ; Peters, Reuben J.; Qi, Xiaoquan3 ; Guo, Juan ; Huang, Luqi
	2021
发表期刊	NATURE COMMUNICATIONS
卷号	12 期号:1
摘要	Tanshinones are the bioactive nor-diterpenoid constituents of the Chinese medicinal herb Danshen (Salvia miltiorrhiza). These groups of chemicals have the characteristic furan D-ring, which differentiates them from the phenolic abietane-type diterpenoids frequently found in the Lamiaceae family. However, how the 14,16-epoxy is formed has not been elucidated. Here, we report an improved genome assembly of Danshen using a highly homozygous genotype. We identify a cytochrome P450 (CYP71D) tandem gene array through gene expansion analysis. We show that CYP71D373 and CYP71D375 catalyze hydroxylation at carbon-16 (C16) and 14,16-ether (hetero)cyclization to form the D-ring, whereas CYP71D411 catalyzes upstream hydroxylation at C20. In addition, we discover a large biosynthetic gene cluster associated with tanshinone production. Collinearity analysis indicates a more specific origin of tanshinones in Salvia genus. It illustrates the evolutionary origin of abietane-type diterpenoids and those with a furan D-ring in Lamiaceae. Salvia miltiorrhiza is a medicinal plant that can produce the bioactive tanshinones. Here, the authors report the improved genome assembly and reveal the possible roles of three CYP71Ds in catalyzing the reactions leading to the formation of the characteristic furan D-ring of transhinones.
学科领域	Multidisciplinary Sciences
DOI	10.1038/s41467-021-20959-1
收录类别	SCI
语种	英语
WOS关键词	PLANT ; BIOSYNTHESIS ; DANSHEN ; PHARMACOKINETICS ; DIVERSIFICATION ; IDENTIFICATION ; CONSTITUENTS ; CHEMISTRY ; GENES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000684845600013
出版者	NATURE PORTFOLIO
文献子类	Article
出版地	BERLIN
EISSN	2041-1723
资助机构	National Natural Science Foundation of China [81822046, 81573532, 81891010, 81891013, 82003904] ; National Key R&D Program of China [2020YFA0908000, 2018YFA0900600] ; Key project at central government level [2060302] ; Fundamental Research Funds of China Academy of Chinese Medical Sciences [ZZ13-YQ-083, ZZ14-YQ-044] ; NIH [GM131885]
作者邮箱	guanghongcui@163.com ; xqi@ibcas.ac.cn ; guojuan@wbgcas.cn ; huangluqi01@126.com
作品OA属性	Green Published, gold
引用统计	被引频次：86[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26681
专题	中科院植物分子生理学重点实验室
作者单位	1.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao Herbs, Beijing, Peoples R China 2.Yunnan Univ Tradit Chinese Med, Coll Pharmaceut Sci, Kunming, Yunnan, Peoples R China 3.Peters, Reuben J.] Iowa State Univ, Roy J Carver Dept Biochem Biophys & Mol Biol, Ames, IA USA 4.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Ma, Ying,Cui, Guanghong,Chen, Tong,et al. Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza[J]. NATURE COMMUNICATIONS,2021,12(1).
APA	Ma, Ying.,Cui, Guanghong.,Chen, Tong.,Ma, Xiaohui.,Wang, Ruishan.,...&Huang, Luqi.(2021).Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza.NATURE COMMUNICATIONS,12(1).
MLA	Ma, Ying,et al."Expansion within the CYP71D subfamily drives the heterocyclization of tanshinones synthesis in Salvia miltiorrhiza".NATURE COMMUNICATIONS 12.1(2021).

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