Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola

doi:10.1038/s41438-021-00533-y

	Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola
	Liu, Guo-Tian ; Wang, Bian-Bian ; Lecourieux, David ; Li, Mei-Jie ; Liu, Ming-Bo ; Liu, Rui-Qi ; Shang, Bo-Xing ; Yin, Xiao ; Wang, Li-Jun ; Lecourieux, Fatma 10; Xu, Yan 7,7
	2021
发表期刊	HORTICULTURE RESEARCH
ISSN	2662-6810
卷号	8 期号:1
摘要	Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii LiuBa-8 (LB) and susceptible V. vinifera Pinot Noir (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H2O2, and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.
学科领域	Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1038/s41438-021-00533-y
收录类别	SCI
语种	英语
WOS关键词	TRANSGENIC TOBACCO PLANTS ; DEFENSE-RELATED GENES ; VITIS-VINIFERA ; PLASMOPARA-VITICOLA ; DISEASE RESISTANCE ; DOWNY MILDEW ; CELL-WALL ; MOLECULAR CHARACTERIZATION ; PHYTOPHTHORA-INFESTANS ; ENHANCED RESISTANCE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000645936200018
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	2052-7276
资助机构	National Key Research and Development Program of China [2018YFD1000300, 2019YFD1002500] ; National Natural Science Foundation of China [31601716, 31972374]
作者邮箱	fatma.ouaked-lecourieux@inrae.fr ; yan.xu@nwafu.edu.cn
作品OA属性	Green Published, gold
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26692
专题	中科院北方资源植物重点实验室
作者单位	1.[Liu, Guo-Tian 2.Wang, Bian-Bian 3.Li, Mei-Jie 4.Liu, Ming-Bo 5.Liu, Rui-Qi 6.Shang, Bo-Xing 7.Yin, Xiao 8.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China 9.Northwest A&F Univ, Key Lab Hort Plant Biol & Germplasm Innovat North, Minist Agr, Yangling, Shaanxi, Peoples R China 10.Lecourieux, David 11.Univ Bordeaux, INRAE, Bordeaux Sci Agro, ISW,UMR1287 EGFV,CNRS, Villenave Dornon, France 12.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Guo-Tian,Wang, Bian-Bian,Lecourieux, David,et al. Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola[J]. HORTICULTURE RESEARCH,2021,8(1).
APA	Liu, Guo-Tian.,Wang, Bian-Bian.,Lecourieux, David.,Li, Mei-Jie.,Liu, Ming-Bo.,...&Xu, Yan.(2021).Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola.HORTICULTURE RESEARCH,8(1).
MLA	Liu, Guo-Tian,et al."Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola".HORTICULTURE RESEARCH 8.1(2021).

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Liu-2021-Proteomic a（1912KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文