An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)

doi:10.3390/ijms22094544

	An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)
	Liu, Jing ; Wang, Juan-Juan ; Wu, Jie ; Wang, Yang ; Liu, Qi ; Liu, Fang-Pu ; Yang, Xia ; Wang, Yin-Zheng
	2021
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:9
摘要	The development of an ideal model plant located at a key phylogenetic node is critically important to advance functional and regulatory studies of key regulatory genes in the evolutionary developmental (evo-devo) biology field. In this study, we selected Chirita pumila in the family Gesneriaceae, a basal group in Lamiales, as a model plant to optimize its genetic transformation system established previously by us through investigating a series of factors and further conduct functional test of the CYC-like floral symmetry gene CpCYC. By transforming a RNAi:CpCYC vector, we successfully achieved the desired phenotypes of upright actinomorphic flowers, which suggest that CpCYC actually determines the establishment of floral zygomorphy and the horizontal orientation of flowers in C. pumila. We also confirmed the activities of CpCYC promoter in dorsal petals, dorsal/lateral staminodes, as well as the pedicel by transferring a CpCYC promoter:GUS vector into C. pumila. Furthermore, we testified the availability of a transient gene expression system using C. pumila mesophyll protoplasts. The improved transformation system together with the inherent biological features would make C. pumila an attractive new model in functional and regulatory studies for a broad range of evo-devo issues.
关键词	Chirita pumila CpCYC evo-devo functional test genetic transformation Gesneriaceae a new model
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms22094544
收录类别	SCI
语种	英语
WOS关键词	AGROBACTERIUM-MEDIATED TRANSFORMATION ; FLORAL ZYGOMORPHY ; EFFICIENT TRANSFORMATION ; NICOTIANA-BENTHAMIANA ; ANTIBIOTIC TIMENTIN ; PLANT-REGENERATION ; REGULATORY CHANGES ; SYMMETRY GENES ; PETAL SHAPE ; CLADE GENES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000650369900001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	National Natural Science Foundation of China [31670217, 31900196, 31970239, 31530003]
作者邮箱	liujing2010@ibcas.ac.cn ; jwang2@mpipz.mpg.de ; daisywujie@mail.tsinghua.edu.cn ; wangyang2017@ibcas.ac.cn ; liuqi2017@ibcas.ac.cn ; liufangpu@ibcas.ac.cn ; yangxia@ibcas.ac.cn ; wangyz@ibcas.ac.cn
作品OA属性	Green Published, gold
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26695
专题	系统与进化植物学国家重点实验室
作者单位	1.[Liu, Jing 2.Wang, Juan-Juan 3.Wu, Jie 4.Wang, Yang 5.Liu, Qi 6.Liu, Fang-Pu 7.Yang, Xia 8.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 9.[Wang, Juan-Juan 10.Univ Chinese Acad Sci, Beijing 101408, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jing,Wang, Juan-Juan,Wu, Jie,et al. An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(9).
APA	Liu, Jing.,Wang, Juan-Juan.,Wu, Jie.,Wang, Yang.,Liu, Qi.,...&Wang, Yin-Zheng.(2021).An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(9).
MLA	Liu, Jing,et al."An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.9(2021).

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