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Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis
Li, Xiaoxia; Yang, Weiguang1; Jia, Junting2; Zhao, Pincang3; Qi, Dongmei; Chen, Shuangyan; Cheng, Li4; Cheng, Liqin; Liu, Gongshe
2021
发表期刊GENES
卷号12期号:12
摘要Sheepgrass is a perennial native grass species in China, and it can tolerate high levels of salt stress with an aggressive and vigorous rhizome system. Many salt-stress-responsive genes have been identified in sheepgrass. In this study, we report the cloning and characterization of a novel salt-induced gene, LcSAIN3 (Leymus chinensis salt-induced 3), from sheepgrass. Expression analysis confirmed that LcSAIN3 was induced by PEG, ABA, and salt treatments, and the expression of LcSAIN3 was significantly increased in salt-tolerant germplasms under salt treatment. Subcellular localization analysis indicated that the GFP-LcSAIN3 protein was mainly localized in the chloroplasts. The heterologous expression of LcSAIN3 in Arabidopsis increased the seed germination rate of transgenic plants under salt, ABA, and mannitol treatments. The seedling survival rate, plant height, and fresh weight of the transgenic plants were higher than those of WT plants under salt stress. The overexpression of LcSAIN3 caused a relatively high accumulation of free proline, enhanced SOD activity, and led to the upregulation of several stress-responsive genes such as AtRD26, AtRD29B, AtSOS1, and AtP5CS1. These results suggest that LcSAIN3 could be a potential target for molecular breeding to improve plants' salt tolerance.
关键词sheepgrass chloroplast salt stress seed germination
学科领域Genetics & Heredity
DOI10.3390/genes12121994
收录类别SCI
语种英语
WOS关键词TRANSCRIPTION FACTOR GENE ; TRANSGENIC ARABIDOPSIS ; ENHANCES TOLERANCE ; LOW-TEMPERATURE ; DREB GENE ; MYB GENE ; DROUGHT ; COLD ; OVEREXPRESSION ; PROTEIN
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000737537700001
出版者MDPI
文献子类Article
出版地BASEL
EISSN2073-4425
资助机构National Natural Science Foundation of China [32071869] ; Key Projects in Science and Technology of the Inner Mongolia [2021ZD0031] ; Strategic Priority Research Program of the Chinese Academy of Science [XDA26030202]
作者邮箱lixx2013@ibcas.ac.cn ; anda580@163.com ; jiajunting123@126.com ; zhaopincang@163.com ; qidm@ibcas.ac.cn ; sychen@ibcas.ac.cn ; cheng1010li@sina.com ; lqcheng@ibcas.ac.cn ; liugs@ibcas.ac.cn
作品OA属性Green Published, gold
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26700
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
2.Helongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163000, Peoples R China
3.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Prov Key Lab Crop Germplasm Resources P, Guangzhou 510640, Peoples R China
4.Hebei Univ Econ & Business, Coll Management Sci & Engn, Shijiazhuang 050062, Hebei, Peoples R China
5.Inst Hulun Buir Forestry & Grassland Sci Res, Hunlunbeier 021008, Peoples R China
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GB/T 7714
Li, Xiaoxia,Yang, Weiguang,Jia, Junting,et al. Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis[J]. GENES,2021,12(12).
APA Li, Xiaoxia.,Yang, Weiguang.,Jia, Junting.,Zhao, Pincang.,Qi, Dongmei.,...&Liu, Gongshe.(2021).Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis.GENES,12(12).
MLA Li, Xiaoxia,et al."Ectopic Expression of a Salt-Inducible Gene, LcSAIN3, from Sheepgrass Improves Seed Germination and Seedling Growth under Salt Stress in Arabidopsis".GENES 12.12(2021).
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