Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP

doi:10.1038/s41467-021-21397-9

IB-CAS > 中科院光生物学重点实验室

	Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP
	Kawakami, Tomoaki ; Yu, Long-Jiang; Liang, Tai ; Okazaki, Koudai ; Madigan, Michael T.; Kimura, Yukihiro 3; Wang-Otomo, Zheng-Yu
	2021
发表期刊	NATURE COMMUNICATIONS
卷号	12 期号:1
摘要	Photosynthetic electron transfers occur through multiple components ranging from small soluble proteins to large integral membrane protein complexes. Co-crystallization of a bacterial photosynthetic electron transfer complex that employs weak hydrophobic interactions was achieved by using high-molar-ratio mixtures of a soluble donor protein (high-potential iron-sulfur protein, HiPIP) with a membrane-embedded acceptor protein (reaction center, RC) at acidic pH. The structure of the co-complex offers a snapshot of a transient bioenergetic event and revealed a molecular basis for thermodynamically unfavorable interprotein electron tunneling. HiPIP binds to the surface of the tetraheme cytochrome subunit in the light-harvesting (LH1) complex-associated RC in close proximity to the low-potential heme-1 group. The binding interface between the two proteins is primarily formed by uncharged residues and is characterized by hydrophobic features. This co-crystal structure provides a model for the detailed study of long-range trans-protein electron tunneling pathways in biological systems. The high potential iron-sulfur (HiPIP) proteins are direct electron donors to the light-harvesting-reaction center complexes (LH1-RC) in photosynthetic beta- and gamma -Proteobacteria. Here, the authors present the 2.9 angstrom crystal structure of the HiPIP-bound LH1-RC complex from the thermophilic purple sulfur bacterium Thermochromatium tepidum and discuss mechanistic implications for the electron transfer pathway.
学科领域	Multidisciplinary Sciences
DOI	10.1038/s41467-021-21397-9
收录类别	SCI
语种	英语
WOS关键词	TETRAHEME CYTOCHROME SUBUNIT ; IRON-SULFUR PROTEIN ; INTERACTION SITE ; BACTERIUM ; BINDING ; C(2) ; THERMOSTABILITY ; THERMODYNAMICS ; COMPENSATION ; FERREDOXIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000621232800023
出版者	NATURE PORTFOLIO
文献子类	Article
出版地	BERLIN
EISSN	2041-1723
资助机构	JSPS KAKENHI [JP16H04174, JP18H05153, JP20H05086, JP20H02856] ; Takeda Science Foundation ; Kurata Memorial Hitachi Science and Technology Foundation, Japan ; grant of KINOU-KYOKA from Institute of Quantum Beam Science of Ibaraki University ; National Key R&D Program of China [2019YFA0904600]
作者邮箱	longer@ibcas.ac.cn ; ykimura@people.kobe-u.ac.jp ; wang@ml.ibaraki.ac.jp
作品OA属性	gold, Green Published
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26704
专题	中科院光生物学重点实验室
作者单位	1.Ibaraki Univ, Fac Sci, Mito, Ibaraki, Japan 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing, Peoples R China 3.Madigan, Michael T.] Southern Illinois Univ, Dept Microbiol, Carbondale, IL USA 4.Kobe Univ, Grad Sch Agr, Dept Agrobiosci, Kobe, Hyogo, Japan
推荐引用方式 GB/T 7714	Kawakami, Tomoaki,Yu, Long-Jiang,Liang, Tai,et al. Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP[J]. NATURE COMMUNICATIONS,2021,12(1).
APA	Kawakami, Tomoaki.,Yu, Long-Jiang.,Liang, Tai.,Okazaki, Koudai.,Madigan, Michael T..,...&Wang-Otomo, Zheng-Yu.(2021).Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP.NATURE COMMUNICATIONS,12(1).
MLA	Kawakami, Tomoaki,et al."Crystal structure of a photosynthetic LH1-RC in complex with its electron donor HiPIP".NATURE COMMUNICATIONS 12.1(2021).

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