High oil accumulation in tuber of yellow nutsedge compared to purple nutsedge is associated with more abundant expression of genes involved in fatty acid synthesis and triacylglycerol storage

doi:10.1186/s13068-021-01909-x

	High oil accumulation in tuber of yellow nutsedge compared to purple nutsedge is associated with more abundant expression of genes involved in fatty acid synthesis and triacylglycerol storage
	Ji, Hongying 1; Liu, Dantong 1; Yang, Zhenle
	2021
发表期刊	BIOTECHNOLOGY FOR BIOFUELS
卷号	14 期号:1
摘要	Background Yellow nutsedge is a unique plant species that can accumulate up to 35% oil of tuber dry weight, perhaps the highest level observed in the tuber tissues of plant kingdom. To gain insight into the molecular mechanism that leads to high oil accumulation in yellow nutsedge, gene expression profiles of oil production pathways involved carbon metabolism, fatty acid synthesis, triacylglycerol synthesis, and triacylglycerol storage during tuber development were compared with purple nutsedge, the closest relative of yellow nutsedge that is poor in oil accumulation. Results Compared with purple nutsedge, high oil accumulation in yellow nutsedge was associated with significant up-regulation of specific key enzymes of plastidial RubisCO bypass as well as malate and pyruvate metabolism, almost all fatty acid synthesis enzymes, and seed-like oil-body proteins. However, overall transcripts for carbon metabolism toward carbon precursor for fatty acid synthesis were comparable and for triacylglycerol synthesis were similar in both species. Two seed-like master transcription factors ABI3 and WRI1 were found to display similar transcript patterns but were expressed at 6.5- and 14.3-fold higher levels in yellow nutsedge than in purple nutsedge, respectively. A weighted gene co-expression network analysis revealed that ABI3 was in strong transcriptional coordination with WRI1 and other key oil-related genes. Conclusions These results implied that pyruvate availability and fatty acid synthesis in plastid, along with triacylglycerol storage in oil bodies, rather than triacylglycerol synthesis in endoplasmic reticulum, are the major factors responsible for high oil production in tuber of yellow nutsedge, and ABI3 most likely plays a critical role in regulating oil accumulation. This study is of significance with regard to understanding the molecular mechanism controlling carbon partitioning toward oil production in oil-rich tuber and provides a valuable reference for enhancing oil accumulation in non-seed tissues of crops through genetic breeding or metabolic engineering.
关键词	Cyperus esculentus Cyperus rotundus Tuber RubisCO bypass Fatty acid synthesis Triacylglycerol storage Oil accumulation Transcriptional control
学科领域	Biotechnology & Applied Microbiology ; Energy & Fuels
DOI	10.1186/s13068-021-01909-x
收录类别	SCI
语种	英语
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000624533100001
出版者	BMC
文献子类	Article
出版地	LONDON
EISSN	1754-6834
资助机构	National Natural Science Foundation of China [31371692] ; Natural Science Foundation of Beijing Municipality [5151001]
作者邮箱	yangzhl@ibcas.ac.cn
作品OA属性	Green Submitted, Green Published, gold
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26709
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ji, Hongying,Liu, Dantong,Yang, Zhenle. High oil accumulation in tuber of yellow nutsedge compared to purple nutsedge is associated with more abundant expression of genes involved in fatty acid synthesis and triacylglycerol storage[J]. BIOTECHNOLOGY FOR BIOFUELS,2021,14(1).
APA	Ji, Hongying,Liu, Dantong,&Yang, Zhenle.(2021).High oil accumulation in tuber of yellow nutsedge compared to purple nutsedge is associated with more abundant expression of genes involved in fatty acid synthesis and triacylglycerol storage.BIOTECHNOLOGY FOR BIOFUELS,14(1).
MLA	Ji, Hongying,et al."High oil accumulation in tuber of yellow nutsedge compared to purple nutsedge is associated with more abundant expression of genes involved in fatty acid synthesis and triacylglycerol storage".BIOTECHNOLOGY FOR BIOFUELS 14.1(2021).

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