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| Isolation and characterization of pathogen causing brown rot in lemon and its control by using ecofriendly botanicals | |
| Liaquat, Fiza; Qunlu, Liu1; Arif, Samiah; Haroon, Urooj2; Saqib, Saddam3,4; Zaman, Wajid3,4; Jianxin, Shi5; Shengquan, Che1; Li, Lv Xin6; Akbar, Mishal7; Munis, Muhammad Farooq Hussain2 | |
| 2021 | |
| Source Publication | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
 |
| ISSN | 0885-5765 |
| Volume | 114 |
| Abstract | Brown rot is a common and devastating disease of different fruits and vegetables. During 2018, a profound brown rot was observed on lemon (Citrus lemon L.) in Islamabad, Pakistan and adjacent areas. This study was designed to identify disease causing pathogen of this disease. Diseased lemon fruit were collected from the orchards and disease causing pathogen was isolated. The morphology of pathogen was examined under stereoscope and compound microscope and the pathogen was identified as Alternaria alternata. For the molecular characterization of this fungus, 18S ribosomal RNA gene was sequenced and its phylogenetic analyses were performed. BLAST analysis revealed 100% similarity with A. alternate NS8, 18S ribosomal RNA gene, partial sequence (MK346034.1). This is the first report of brown rot of lemon, caused by A. alternata in Pakistan. For ecofriendly control of this disease, extracts of indigenous medicinal plants including Justacia adhatoda, Azadriachta indica, Foeniculum vulgare and Mentha spicata were used. In these plant extracts, the presence of various functional groups such as alcohols, carboxylic acids, esters, alkanes and alkenes was revealed by Fourier transform infrared spectroscopy (FTIR). Both in vitro and in vivo assay showed the significant role of aqueous plant extracts to suppress the growth of A. alternata. Among these phytoextracts, A. indica proved to be the most efficient in suppressing the growth of the pathogen and controlling disease. Findings of this study suggest the use of these plant extracts for environment friendly control of fruit rot disease. |
| Keyword | A alternata Brown rot Citrus limon L 18S rDNA Phyto extracts |
| Subject Area | Plant Sciences |
| DOI | 10.1016/j.pmpp.2021.101639 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Keyword | ALTERNARIA-ALTERNATA ; 1ST REPORT ; LEAF-SPOT ; PLANT |
| WOS Research Area | Science Citation Index Expanded (SCI-EXPANDED) |
| WOS ID | WOS:000652019700007 |
| Publisher | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| Subtype | Article |
| Publication Place | LONDON |
| Funding Organization | University Research Fund (URF) QuaidiAzam University, Islamabad, Pakistan ; Shanghai Sciences and Technology Commission [18DZ2283500] |
| Corresponding Author Email | fiza.liaquat@bs.qau.edu.pk ; liuql@sjtu.edu.cn ; Samiaharif24@gmail.com ; uharoonsheikh@gmail.com ; saddamsaqib.qau@gmail.com ; shangla123@gmail.com ; jianxin.shi@sjtu.edu.cn ; chsq@sjtu.edu.cn ; tkdyun@163.com ; mishalakbar222@gmail.com ; munis@qau.edu.pk |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ibcas.ac.cn/handle/2S10CLM1/26710 |
| Collection | 系统与进化植物学国家重点实验室 |
| Affiliation | 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Design, Dept Landscape Architecture, Shanghai 200240, Peoples R China 3.Quaid I Azam Univ, Fac Biol Sci, Dept Plant Sci, Islamabad 45320, Pakistan 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Joint Int Res Lab Metab & Dev Sci, Shanghai 200240, Peoples R China 7.Shanghai Acad Landscape Architecture Sci & Planni, Shanghai 200232, Peoples R China 8.Army Med Coll Rawalpindi, Rawalpindi 45320, Pakistan |
| Recommended Citation GB/T 7714 | Liaquat, Fiza,Qunlu, Liu,Arif, Samiah,et al. Isolation and characterization of pathogen causing brown rot in lemon and its control by using ecofriendly botanicals[J]. PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY,2021,114. |
| APA | Liaquat, Fiza.,Qunlu, Liu.,Arif, Samiah.,Haroon, Urooj.,Saqib, Saddam.,...&Munis, Muhammad Farooq Hussain.(2021).Isolation and characterization of pathogen causing brown rot in lemon and its control by using ecofriendly botanicals.PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY,114. |
| MLA | Liaquat, Fiza,et al."Isolation and characterization of pathogen causing brown rot in lemon and its control by using ecofriendly botanicals".PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY 114(2021). |
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| Isolation_and_charac（5519KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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