Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis

doi:10.1038/s41438-020-00458-y

	Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis
	Huang, Hui 1; Liang, Juan ; Tan, Qi ; Ou, Linfeng ; Li, Xiaolin 2; Zhong, Caihong ; Huang, Huilin ; Moller, Ian Max 3; Wu, Xianjin ; Song, Songquan 4
	2021
发表期刊	HORTICULTURE RESEARCH
ISSN	2662-6810
卷号	8 期号:1
摘要	Akebia trifoliata subsp. australis is a well-known medicinal and potential woody oil plant in China. The limited genetic information available for A. trifoliata subsp. australis has hindered its exploitation. Here, a high-quality chromosome-level genome sequence of A. trifoliata subsp. australis is reported. The de novo genome assembly of 682.14Mb was generated with a scaffold N50 of 43.11Mb. The genome includes 25,598 protein-coding genes, and 71.18% (485.55Mb) of the assembled sequences were identified as repetitive sequences. An ongoing massive burst of long terminal repeat (LTR) insertions, which occurred similar to 1.0 million years ago, has contributed a large proportion of LTRs in the genome of A. trifoliata subsp. australis. Phylogenetic analysis shows that A. trifoliata subsp. australis is closely related to Aquilegia coerulea and forms a clade with Papaver somniferum and Nelumbo nucifera, which supports the well-established hypothesis of a close relationship between basal eudicot species. The expansion of UDP-glucoronosyl and UDP-glucosyl transferase gene families and beta-amyrin synthase-like genes and the exclusive contraction of terpene synthase gene families may be responsible for the abundant oleanane-type triterpenoids in A. trifoliata subsp. australis. Furthermore, the acyl-ACP desaturase gene family, including 12 stearoyl-acyl-carrier protein desaturase (SAD) genes, has expanded exclusively. A combined transcriptome and fatty-acid analysis of seeds at five developmental stages revealed that homologs of SADs, acyl-lipid desaturase omega fatty acid desaturases (FADs), and oleosins were highly expressed, consistent with the rapid increase in the content of fatty acids, especially unsaturated fatty acids. The genomic sequences of A. trifoliata subsp. australis will be a valuable resource for comparative genomic analyses and molecular breeding.
学科领域	Plant Sciences ; Genetics & Heredity ; Horticulture
DOI	10.1038/s41438-020-00458-y
收录类别	SCI
语种	英语
WOS关键词	READ ALIGNMENT ; OIL BODIES ; GENES ; IDENTIFICATION ; ANNOTATION ; RETROTRANSPOSONS ; CAROTENOIDS ; OLEOSINS ; SAPONINS ; DATABASE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000616721100015
出版者	OXFORD UNIV PRESS INC
文献子类	Article
出版地	CARY
EISSN	2052-7276
资助机构	Natural Science Foundation of Hunan Province [2019JJ50475] ; Key Scientific Research Projects of Hunan Education Department [18A448] ; Foundation of Hunan Double First-rate Discipline Construction Projects of Bioengineering and Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province ; National Science Foundation [81874334]
作者邮箱	sqsong@ibcas.ac.cn
作品OA属性	gold, Green Published
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26718
专题	中科院北方资源植物重点实验室
作者单位	1.Huaihua Univ, Coll Biol & Food Engn, Key Lab Res & Utilizat Ethnomed Plant Resources H, Huaihua 418000, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 3.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao di Herbs, Beijing 100700, Peoples R China 4.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark 5.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Huang, Hui,Liang, Juan,Tan, Qi,et al. Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis[J]. HORTICULTURE RESEARCH,2021,8(1).
APA	Huang, Hui.,Liang, Juan.,Tan, Qi.,Ou, Linfeng.,Li, Xiaolin.,...&Song, Songquan.(2021).Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis.HORTICULTURE RESEARCH,8(1).
MLA	Huang, Hui,et al."Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis".HORTICULTURE RESEARCH 8.1(2021).

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