On the Species Delimitation of the Maddenia Group of Prunus (Rosaceae): Evidence From Plastome and Nuclear Sequences and Morphology

doi:10.3389/fpls.2021.743643

	On the Species Delimitation of the Maddenia Group of Prunus (Rosaceae): Evidence From Plastome and Nuclear Sequences and Morphology
	Su, Na ; Liu, Bin-bin ; Wang, Jun-ru ; Tong, Ru-chang ; Ren, Chen 4,5; Chang, Zhao-yang ; Zhao, Liang 1; Potter, Daniel 6; Wen, Jun 3
	2021
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	12
摘要	The recognition, identification, and differentiation of closely related plant species present significant and notorious challenges to taxonomists. The Maddenia group of Prunus, which comprises four to seven species, is an example of a group in which species delimitation and phylogenetic reconstruction have been difficult, due to the lack of clear morphological distinctions, limited sampling, and low informativeness of molecular evidence. Thus, the precise number of species in the group and the relationships among them remain unclear. Here, we used genome skimming to generate the DNA sequence data for 22 samples, including 17 Maddenia individuals and five outgroups in Amygdaloideae of Rosaceae, from which we assembled the plastome and 446 single-copy nuclear (SCN) genes for each sample. The phylogenetic relationships of the Maddenia group were then reconstructed using both concatenated and coalescent-based methods. We also identified eight highly variable regions and detected simple sequence repeats (SSRs) and repeat sequences in the Maddenia species plastomes. The phylogenetic analysis based on the complete plastomes strongly supported three main subclades in the Maddenia group of Prunus, while five subclades were recognized based on the nuclear tree. The phylogenetic network analysis detected six hybridization events. Integrating the nuclear and morphological evidence, we proposed to recognize five species within the Maddenia group, i.e., Prunus fujianensis, P. himalayana, P. gongshanensis, P. hypoleuca, and P. hypoxantha. Within this group, the first three species are well-supported, while the gene flow occurring throughout the Maddenia group seems to be especially frequent between P. hypoleuca and P. hypoxantha, eroding the barrier between them. The phylogenetic trees based on eight concatenated hypervariable regions had a similar topology with the complete plastomes, showing their potential as molecular markers and effective barcodes for further phylogeographic studies on Maddenia.
关键词	Maddenia Prunus Rosaceae barcoding chloroplast genome single-copy nuclear genes species delimitation
学科领域	Plant Sciences
DOI	10.3389/fpls.2021.743643
收录类别	SCI
语种	英语
WOS关键词	COMPLETE CHLOROPLAST GENOME ; PHYLOGENETIC ANALYSIS ; READ ALIGNMENT ; DNA BARCODES ; MARKERS ; SOFTWARE ; MICROSATELLITES ; DIVERSIFICATION ; MITOCHONDRIAL ; INFERENCES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000726165000001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
作者邮箱	biology_zhaoliang@126.com
作品OA属性	gold, Green Published
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26729
专题	系统与进化植物学国家重点实验室
作者单位	1.Northwest A&F Univ, Coll Life Sci, Yangling, Shaanxi, Peoples R China 2.Herbarium Northwest A&F Univ, Yangling, Shaanxi, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing, Peoples R China 4.Smithsonian Inst, Natl Museum Nat Hist, Dept Bot, MRC 166, Washington, DC 20560 USA 5.Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou, Peoples R China 6.Chinese Acad Sci, South China Bot Garden, Core Bot Gardens, Ctr Conservat Biol, Guangzhou, Peoples R China 7.Univ Calif Davis, Dept Plant Sci, MS2, Davis, CA 95616 USA
推荐引用方式 GB/T 7714	Su, Na,Liu, Bin-bin,Wang, Jun-ru,et al. On the Species Delimitation of the Maddenia Group of Prunus (Rosaceae): Evidence From Plastome and Nuclear Sequences and Morphology[J]. FRONTIERS IN PLANT SCIENCE,2021,12.
APA	Su, Na.,Liu, Bin-bin.,Wang, Jun-ru.,Tong, Ru-chang.,Ren, Chen.,...&Wen, Jun.(2021).On the Species Delimitation of the Maddenia Group of Prunus (Rosaceae): Evidence From Plastome and Nuclear Sequences and Morphology.FRONTIERS IN PLANT SCIENCE,12.
MLA	Su, Na,et al."On the Species Delimitation of the Maddenia Group of Prunus (Rosaceae): Evidence From Plastome and Nuclear Sequences and Morphology".FRONTIERS IN PLANT SCIENCE 12(2021).

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