Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata

doi:10.3389/fpls.2021.743518

	Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata
	Shen, Guoan ; Wu, Ranran 1; Xia, Yaying 1,2; Pang, Yongzhen 2
	2021
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	12
摘要	Kudzu, Pueraria lobata, is a traditional Chinese food and medicinal herb that has been commonly used since ancient times. Kudzu roots are rich sources of isoflavonoids, e.g., puerarin, with beneficial effects on human health. To gain global information on the isoflavonoid biosynthetic regulation network in kudzu, de novo transcriptome sequencings were performed using two genotypes of kudzu with and without puerarin accumulation in roots. RNAseq data showed that the genes of the isoflavonoid biosynthetic pathway were significantly represented in the upregulated genes in the kudzu with puerarin. To discover regulatory genes, 105, 112, and 143 genes encoding MYB, bHLH, and WD40 transcription regulators were identified and classified, respectively. Among them, three MYB, four bHLHs, and one WD40 gene were found to be highly identical to their orthologs involved in flavonoid biosynthesis in other plants. Notably, the expression profiles of PlMYB1, PlHLH3-4, and PlWD40-1 genes were closely correlated with isoflavonoid accumulation profiles in different tissues and cell cultures of kudzu. Over-expression of PlMYB1 in Arabidopsis thaliana significantly increased the accumulation of anthocyanins in leaves and proanthocyanidins in seeds, by activating AtDFR, AtANR, and AtANS genes. Our study provided valuable comparative transcriptome information for further identification of regulatory or structural genes involved in the isoflavonoid pathway in P. lobata, as well as for bioengineering of bioactive isoflavonoid compounds.
关键词	Pueraria lobata transcriptome sequencing transcription factors MYB isoflavonoid biosynthesis
学科领域	Plant Sciences
DOI	10.3389/fpls.2021.743518
收录类别	SCI
语种	英语
WOS关键词	TISSUE-SPECIFIC ANTHOCYANIN ; PROANTHOCYANIDIN BIOSYNTHESIS ; ECTOPIC EXPRESSION ; REGULATORY GENE ; PETUNIA ENCODES ; DOMAIN PROTEIN ; REPEAT PROTEIN ; GENOME-WIDE ; ARABIDOPSIS ; BHLH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000710921300001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Science Foundation of China [31570306] ; Key Program of the National Natural Science Foundation of China [U20A2004] ; CAMS Innovation Fund for Medical Sciences (CIFMS) [2017-I2M-3-013] ; Agricultural Science and Technology Innovation Program [ASTIP-IAS10]
作者邮箱	pangyongzhen@caas.cn
作品OA属性	Green Published, gold
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26730
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Med Sci, Peking Union Med Coll, Inst Med Plant Dev, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing Bot Garden, Beijing, Peoples R China 3.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Shen, Guoan,Wu, Ranran,Xia, Yaying,et al. Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata[J]. FRONTIERS IN PLANT SCIENCE,2021,12.
APA	Shen, Guoan,Wu, Ranran,Xia, Yaying,&Pang, Yongzhen.(2021).Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata.FRONTIERS IN PLANT SCIENCE,12.
MLA	Shen, Guoan,et al."Identification of Transcription Factor Genes and Functional Characterization of PlMYB1 From Pueraria lobata".FRONTIERS IN PLANT SCIENCE 12(2021).

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