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LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin
Liu, Jinyue1,2,3; Xia, Yaying1,2,3; Jiang, Wenbo; Shen, Guoan4; Pang, Yongzhen
2021
发表期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
卷号12
摘要Legume plants are rich in prenylated flavonoid compounds, which play an important role in plant defense and human health. In the present study, we identified a prenyltransferase (PT) gene, named LaPT2, in white lupin (Lupinus albus), which shows a high identity and close relationship with the other known PT genes involved in flavonoid prenylation in planta. The recombinant LaPT2 protein expressed in yeast cells exhibited a relatively strong activity toward several flavonols (e.g., kaempferol, quercetin, and myricetin) and a relatively weak activity toward flavanone (naringenin). In addition, the recombinant LaPT2 protein was also active toward several other types of flavonoids, including galangin, morin, 5-deoxyquercetin, 4'-O-methylkaempferol, taxifolin, and aromadendrin, with distinct enzymatic affinities. The LaPT2 gene was preferentially expressed in the roots, which is consistent with the presence of prenylated flavonoid kaempferol in the roots. Moreover, we found that the expression level of LaPT2 paralleled with those of LaF3H1 and LaFLS2 genes that were relatively higher in roots and lower in leaves, suggesting that they were essential for the accumulation of prenylated flavonoid kaempferol in roots. The deduced full-length LaPT2 protein and its signal peptide fused with a green fluorescent protein (GFP) are targeted to plastids in the Arabidopsis thaliana protoplast. Our study demonstrated that LaPT2 from white lupin is responsible for the biosynthesis of prenylated flavonoids, in particular flavonols, which could be utilized as phytoalexin for plant defense and bioactive flavonoid compounds for human health.
关键词white lupin flavonoids prenyltransferase LaPT2 kaempferol prenylation
学科领域Plant Sciences
DOI10.3389/fpls.2021.673337
收录类别SCI
语种英语
WOS关键词SOPHORA-FLAVESCENS ; MOLECULAR CHARACTERIZATION ; PRENYLATED ISOFLAVONOIDS ; BIOSYNTHESIS ; GLYCEOLLIN ; FLAVANONE ; BIOLOGY ; CLONING ; ROOTS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000665741300001
出版者FRONTIERS MEDIA SA
文献子类Article
出版地LAUSANNE
资助机构National Nature Science Foundation of China [U1906201] ; Basic Scientific Research Funding of CAAS [2019-YWF-YB-06] ; Central Publicinterest Scientific Institution Basal Research Fund [Y2020GH01-3]
作者邮箱pangyongzhen@caas.cn
作品OA属性gold, Green Published
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26731
专题中科院北方资源植物重点实验室
作者单位1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Inst Med Plant Dev, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Liu, Jinyue,Xia, Yaying,Jiang, Wenbo,et al. LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin[J]. FRONTIERS IN PLANT SCIENCE,2021,12.
APA Liu, Jinyue,Xia, Yaying,Jiang, Wenbo,Shen, Guoan,&Pang, Yongzhen.(2021).LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin.FRONTIERS IN PLANT SCIENCE,12.
MLA Liu, Jinyue,et al."LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin".FRONTIERS IN PLANT SCIENCE 12(2021).
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