Dietary Moutan Cortex Radicis Improves Serum Antioxidant Capacity and Intestinal Immunity and Alters Colonic Microbiota in Weaned Piglets

doi:10.3389/fnut.2021.679129

	Dietary Moutan Cortex Radicis Improves Serum Antioxidant Capacity and Intestinal Immunity and Alters Colonic Microbiota in Weaned Piglets
	Bai, Miaomiao 1,2,3,4,5; Liu, Hongnan 1,2,3,4; Wang, Shanshan 1,2,3,4; Shu, Qingyan ; Xu, Kang 1,2,3,4; Zhou, Jian 1,2,3,4; Xiong, Xia 1,2,3,4; Huang, Ruilin 1,2,3,4; Deng, Jinping 5; Yin, Yulong 1,2,3,4,5; Liu, Zheng'an
	2021
发表期刊	FRONTIERS IN NUTRITION
ISSN	2296-861X
卷号	8
摘要	Background: Moutan cortex radicis (MCR), as a common traditional Chinese medicine, has been widely used as an antipyretic, antiseptic, and anti-inflammatory agent in China. Objectives: This study aimed to investigate the effects of dietary MCR supplementation on the antioxidant capacity and intestinal health of the pigs and to explore whether MCR exerts positive effects on intestinal health via regulating nuclear factor kappa-B (NF-kappa B) signaling pathway and intestinal microbiota. Methods: MCR powder was identified by LC-MS analysis. Selected 32 weaned piglets (21 d of age, 6.37 +/- 0.10 kg average BW) were assigned (8 pens/diet, 1 pig/pen) to 4 groups and fed with a corn-soybean basal diet supplemented with 0, 2,000, 4,000, and 8,000 mg/kg MCR for 21 d. After the piglets were sacrificed, antioxidant indices, histomorphology examination, and inflammatory signaling pathway expression were assessed. The 16s RNA sequencing was used to analyze the effects of MCR on the intestinal microbiota structure of piglets. Results: Supplemental 4,000 mg/kg MCR significantly increased (P < 0.05) the average daily weight gain (ADG), average daily feed intake (ADFI), total antioxidative capability, colonic short-chain fatty acids (SCFA) concentrations, and the crypt depth in the jejunum but decreased (P < 0.05) the mRNA expression levels of interferon gamma, tumor necrosis factor-alpha, interleukin-1 beta, inhibiting kappa-B kinase beta (IKK beta), inhibiting nuclear factor kappa-B (I kappa B alpha), and NF-kappa B in the jejunum and ileum. Microbiota sequencing identified that MCR supplementation significantly increased the microbial richness indices (Chao1, ACE, and observed species, P < 0.05) and the relative abundances of Firmicutes and Lactobacillus (P < 0.05), decreased the relative abundances of Bacteroides, Parabacteroides, unidentified_Lachnospiraceae, and Enterococcus (P < 0.05) and had no significant effects on the diversity indices (Shannon and Simpson, P > 0.05). Microbial metabolic phenotypes analysis also showed that the richness of aerobic bacteria and facultative anaerobic bacteria, oxidative stress tolerance, and biofilm forming were significantly increased (P < 0.05), and the richness of anaerobic bacteria and pathogenic potential of gut microbiota were reduced (P < 0.05) by MCR treatment. Regression analysis showed that the optimalMCR supplemental level for growth performance, serum antioxidant capacity, and intestinal health of weaned piglets was 3,420 similar to 4,237 mg/kg. Conclusions: MCR supplementation improved growth performance and serum antioxidant capacity, and alleviated intestinal inflammation by inhibiting the IKK beta/I kappa B alpha/NF-kappa B signaling pathway and affecting intestinal microbiota in weaned piglets.
关键词	Moutan cortex radicis antioxidant capacity intestinal microbiota NF-kappa B signaling weaned piglets
学科领域	Nutrition & Dietetics
DOI	10.3389/fnut.2021.679129
收录类别	SCI
语种	英语
WOS关键词	INDUCED OXIDATIVE STRESS ; NF-KAPPA-B ; GUT MICROBIOME ; EPITHELIAL-CELLS ; BARRIER FUNCTION ; GENE-EXPRESSION ; WEANING STRESS ; INHIBITION ; RATS ; SUPPLEMENTATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000668627000001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
资助机构	National Natural Science Foundation of China [32072741] ; Innovation Team in Key Area Innovation Team of Physiology and Metabolism and Body Health in Pig [2019RS3022] ; Science and Technology Program of Changsha [kq1907074] ; Huxiang Youth Talent Program of Hunan Province [2018RS3110] ; Youth Innovation Promotion Association, CAS [2019356] ; Special Funds for the Construction of Innovative Provinces in Hunan [2019RS1068, 2019NK2193] ; China Agriculture Research System [CARS-35]
作者邮箱	liuhn@isa.ac.cn ; shuqy@ibcas.ac.cn ; dengjinping@scau.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26734
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Subtrop Agr, Minist Agr, Hunan Prov Key Lab Anim Nutr Physiol & Metab Proc, Changsha, Peoples R China 2.Chinese Acad Sci, Inst Subtrop Agr, Minist Agr, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Changsha, Peoples R China 3.Chinese Acad Sci, Inst Subtrop Agr, Minist Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Peoples R China 4.Chinese Acad Sci, Inst Subtrop Agr, Minist Agr, Hunan Prov Engn Res Ctr Hlth Livestock & Poult Pr, Changsha, Peoples R China 5.Chinese Acad Sci, Inst Subtrop Agr, Minist Agr, Sci Observing & Expt Stn Anim Nutr & Feed Sci Sou, Changsha, Peoples R China 6.South China Agr Univ, Coll Anim Sci, Guangzhou, Peoples R China 7.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Key Lab Plant Resources, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Bai, Miaomiao,Liu, Hongnan,Wang, Shanshan,et al. Dietary Moutan Cortex Radicis Improves Serum Antioxidant Capacity and Intestinal Immunity and Alters Colonic Microbiota in Weaned Piglets[J]. FRONTIERS IN NUTRITION,2021,8.
APA	Bai, Miaomiao.,Liu, Hongnan.,Wang, Shanshan.,Shu, Qingyan.,Xu, Kang.,...&Liu, Zheng'an.(2021).Dietary Moutan Cortex Radicis Improves Serum Antioxidant Capacity and Intestinal Immunity and Alters Colonic Microbiota in Weaned Piglets.FRONTIERS IN NUTRITION,8.
MLA	Bai, Miaomiao,et al."Dietary Moutan Cortex Radicis Improves Serum Antioxidant Capacity and Intestinal Immunity and Alters Colonic Microbiota in Weaned Piglets".FRONTIERS IN NUTRITION 8(2021).

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