Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice

doi:10.1016/j.foodchem.2021.129046

	Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice
	Xing, Mengyang 1; Chen, Yong ; Li, Boqiang ; Tian, Shiping1
	2021
发表期刊	FOOD CHEMISTRY
ISSN	0308-8146
卷号	348
摘要	Biodegradation based on microbial enzymes is considered to be one of the promising ways for controlling patulin contamination. However, few patulin degrading enzymes have been isolated and characterized until now. Here, a short-chain dehydrogenase/reductase (SDR) gene, CgSDR, was cloned from a yeast strain Candida guilliermondii, and expressed in Escherichia coli. The expression of CgSDR conferred a strong patulin tolerance and degradation ability to E. coli, and purified CgSDR could transform patulin into E-ascladiol in vitro with NADPH as a coenzyme. Moreover, addition of CgSDR at 150 mu g/mL could reduce 80% of patulin in apple juice and the biodegradation process did not affect the quality of the apple juice. A molecular docking analysis and site-directed mutagenesis indicated that CgSDR might interact with patulin via VAL188 as an active binding sites. The findings provide new insights for developing enzymic formulations for mycotoxin detoxification in fruit derived products.
关键词	Patulin Degradation Short-chain dehydrogenase reductase Apple juice
学科领域	Chemistry, Applied ; Food Science & Technology ; Nutrition & Dietetics
DOI	10.1016/j.foodchem.2021.129046
收录类别	SCI
语种	英语
WOS关键词	MYCOTOXIN PATULIN ; SUPERFAMILY ; SDR ; CLASSIFICATION ; DEGRADATION ; PH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000623049400012
出版者	ELSEVIER SCI LTD
文献子类	Article
出版地	OXFORD
EISSN	1873-7072
资助机构	National Natural Science Foundation of China [31930086, 31722043] ; National Key R&D Program of China [2016YFD0400902] ; Youth Innovation Promotion Association, CAS [Y201919]
作者邮箱	bqli@ibcas.ac.cn ; tsp@ibcas.ac.cn
引用统计	被引频次：41[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26764
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xing, Mengyang,Chen, Yong,Li, Boqiang,et al. Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice[J]. FOOD CHEMISTRY,2021,348.
APA	Xing, Mengyang,Chen, Yong,Li, Boqiang,&Tian, Shiping.(2021).Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice.FOOD CHEMISTRY,348.
MLA	Xing, Mengyang,et al."Characterization of a short-chain dehydrogenase/reductase and its function in patulin biodegradation in apple juice".FOOD CHEMISTRY 348(2021).

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