Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance

doi:10.3390/ijms22179175

	Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance
	Ayaz, Asma ; Huang, Haodong ; Zheng, Minglu ; Zaman, Wajid 1; Li, Donghai ; Saqib, Saddam 2,3; Zhao, Huayan ; Lu, Shiyou
	2021
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:17
摘要	Cutin and wax are the main precursors of the cuticle that covers the aerial parts of plants and provide protection against biotic and abiotic stresses. Long-chain acyl-CoA synthetases (LACSs) play diversified roles in the synthesis of cutin, wax, and triacylglycerol (TAG). Most of the information concerned with LACS functions is obtained from model plants, whereas the roles of LACS genes in Glycine max are less known. Here, we have identified 19 LACS genes in Glycine max, an important crop plant, and further focused our attention on 4 LACS2 genes (named as GmLACS2-1, 2, 3, 4, respectively). These GmLACS2 genes display different expression patterns in various organs and also show different responses to abiotic stresses, implying that these genes might play diversified functions during plant growth and against stresses. To further identify the role of GmLACS2-3, greatly induced by abiotic stresses, we transformed a construct containing its full length of coding sequence into Arabidopsis. The expression of GmLACS2-3 in an Arabidopsis atlacs2 mutant greatly suppressed its phenotype, suggesting it plays conserved roles with that of AtLACS2. The overexpression of GmLACS2-3 in wild-type plants significantly increased the amounts of cutin and suberin but had little effect on wax amounts, indicating the specific role of GmLACS2-3 in the synthesis of cutin and suberin. In addition, these GmLACS2-3 overexpressing plants showed enhanced drought tolerance. Taken together, our study deepens our understanding of the functions of LACS genes in different plants and also provides a clue for cultivating crops with strong drought resistance.
关键词	long-chain acyl-CoA synthetases Glycine max abiotic stresses cutin cuticular wax suberin
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms22179175
收录类别	SCI
语种	英语
WOS关键词	ACYL-COENZYME ; WAX ; GENE ; IDENTIFICATION ; SYNTHETASE ; DEFICIENCY ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000694265900001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	Natural Science Foundation of China [32070282]
作者邮箱	asmaayaz@bs.qau.edu.pk ; haodonghuang@stu.hubu.edu.cn ; 202021107010975@stu.hubu.edu.cn ; shangla123@gmail.com ; lidonghail7@mails.ucas.edu.cn ; saddamsaqib.qau@gmail.com ; huayanzhao@hubu.edu.cn ; shiyoulu@hubu.edu.cn
作品OA属性	gold, Green Published
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26770
专题	系统与进化植物学国家重点实验室
作者单位	1.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China 2.Chinese Acad Sci, Lushan Bot Garden, Nanchang 332900, Jiangxi, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Ayaz, Asma,Huang, Haodong,Zheng, Minglu,et al. Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(17).
APA	Ayaz, Asma.,Huang, Haodong.,Zheng, Minglu.,Zaman, Wajid.,Li, Donghai.,...&Lu, Shiyou.(2021).Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(17).
MLA	Ayaz, Asma,et al."Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.17(2021).

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