IB-CAS  > 中科院光生物学重点实验室
The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis
Zhang, Jing1,2; Bai, Zechen2; Ouyang, Min3; Xu, Xiumei1; Xiong, Haibo2; Wang, Qiang1; Grimm, Bernhard4; Rochaix, Jean-David; Zhang, Lixin1
2021
发表期刊EMBO JOURNAL
ISSN0261-4189
卷号40期号:13
摘要Fe-S clusters are ancient, ubiquitous and highly essential prosthetic groups for numerous fundamental processes of life. The biogenesis of Fe-S clusters is a multistep process including iron acquisition, sulfur mobilization, and cluster formation. Extensive studies have provided deep insights into the mechanism of the latter two assembly steps. However, the mechanism of iron utilization during chloroplast Fe-S cluster biogenesis is still unknown. Here we identified two Arabidopsis DnaJ proteins, DJA6 and DJA5, that can bind iron through their conserved cysteine residues and facilitate iron incorporation into Fe-S clusters by interactions with the SUF (sulfur utilization factor) apparatus through their J domain. Loss of these two proteins causes severe defects in the accumulation of chloroplast Fe-S proteins, a dysfunction of photosynthesis, and a significant intracellular iron overload. Evolutionary analyses revealed that DJA6 and DJA5 are highly conserved in photosynthetic organisms ranging from cyanobacteria to higher plants and share a strong evolutionary relationship with SUFE1, SUFC, and SUFD throughout the green lineage. Thus, our work uncovers a conserved mechanism of iron utilization for chloroplast Fe-S cluster biogenesis.
关键词assembly chloroplast evolution iron donor iron– sulfur cluster
学科领域Biochemistry & Molecular Biology ; Cell Biology
DOI10.15252/embj.2020106742
收录类别SCI
语种英语
WOS关键词ARABIDOPSIS-THALIANA ; OXIDATIVE STRESS ; DEFICIENCY RESPONSES ; SCAFFOLD PROTEIN ; GENE-EXPRESSION ; 4FE-4S CLUSTERS ; MATURATION ; SYSTEM ; HOMEOSTASIS ; FERRITINS
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号WOS:000640181700001
出版者WILEY
文献子类Article
出版地HOBOKEN
EISSN1460-2075
资助机构National Key Research and Development Program [2020YFA0907600] ; 111 Project [D16014] ; Outstanding Talents Fund of Henan University, China
作者邮箱zhanglixin@henu.edu.cn
作品OA属性Green Published
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ibcas.ac.cn/handle/2S10CLM1/26783
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing, Peoples R China
2.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adapt & Improvement, Kaifeng, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan, Peoples R China
5.Humboldt Univ, Inst Biol Plant Physiol, Berlin, Germany
6.Univ Geneva, Dept Mol Biol, Geneva, Switzerland
7.Univ Geneva, Dept Plant Biol, Geneva, Switzerland
推荐引用方式
GB/T 7714
Zhang, Jing,Bai, Zechen,Ouyang, Min,et al. The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis[J]. EMBO JOURNAL,2021,40(13).
APA Zhang, Jing.,Bai, Zechen.,Ouyang, Min.,Xu, Xiumei.,Xiong, Haibo.,...&Zhang, Lixin.(2021).The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis.EMBO JOURNAL,40(13).
MLA Zhang, Jing,et al."The DnaJ proteins DJA6 and DJA5 are essential for chloroplast iron-sulfur cluster biogenesis".EMBO JOURNAL 40.13(2021).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Applet_2023-1-17_167(3774KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Jing]的文章
[Bai, Zechen]的文章
[Ouyang, Min]的文章
百度学术
百度学术中相似的文章
[Zhang, Jing]的文章
[Bai, Zechen]的文章
[Ouyang, Min]的文章
必应学术
必应学术中相似的文章
[Zhang, Jing]的文章
[Bai, Zechen]的文章
[Ouyang, Min]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Applet_2023-1-17_167392116128113.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。