TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth

doi:10.15252/embj.2021108684

	TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth
	Yan, Jiapei ; Li, Shibai 1; Kim, Yeon Jeong ; Zeng, Qingning ; Radziejwoski, Amandine ; Wang, Lei3 ; Nomura, Yuko 4; Nakagami, Hirofumi 4,5; Somers, David E.
	2021
发表期刊	EMBO JOURNAL
ISSN	0261-4189
卷号	40 期号:24
摘要	Plant photoperiodic growth is coordinated by interactions between circadian clock and light signaling networks. How post-translational modifications of clock proteins affect these interactions to mediate rhythmic growth remains unclear. Here, we identify five phosphorylation sites in the Arabidopsis core clock protein TIMING OF CAB EXPRESSION 1 (TOC1) which when mutated to alanine eliminate detectable phosphorylation. The TOC1 phospho-mutant fails to fully rescue the clock, growth, and flowering phenotypes of the toc1 mutant. Further, the TOC1 phospho-mutant shows advanced phase, a faster degradation rate, reduced interactions with PHYTOCHROME-INTERACTING FACTOR 3 (PIF3) and HISTONE DEACETYLASE 15 (HDA15), and poor binding at pre-dawn hypocotyl growth-related genes (PHGs), leading to a net de-repression of hypocotyl growth. NUCLEAR FACTOR Y subunits B and C (NF-YB/C) stabilize TOC1 at target promoters, and this novel trimeric complex (NF-TOC1) acts as a transcriptional co-repressor with HDA15 to inhibit PIF-mediated hypocotyl elongation. Collectively, we identify a molecular mechanism suggesting how phosphorylation of TOC1 alters its phase, stability, and physical interactions with co-regulators to precisely phase PHG expression to control photoperiodic hypocotyl growth.
关键词	circadian NUCLEAR FACTOR Y phosphorylation photomorphogenesis TOC1
学科领域	Biochemistry & Molecular Biology ; Cell Biology
DOI	10.15252/embj.2021108684
收录类别	SCI
语种	英语
WOS关键词	ARABIDOPSIS CIRCADIAN CLOCK ; POSTTRANSLATIONAL REGULATION ; COINCIDENCE MECHANISM ; MOLECULAR-BASES ; LIGHT ; REGULATOR ; CONSTANS ; PHYTOCHROMES ; ELONGATION ; ZEITLUPE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000713612500001
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1460-2075
资助机构	National Institutes of Health [R01GM093285, R35GM136400] ; Next-Generation BioGreen21 Program, the Rural Development Administration, Republic of Korea [PJ01327305] ; World Class University Program of South Korea, NRF, MEST [R31-2008-000-10105-0]
作者邮箱	somers.24@osu.edu
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26785
专题	中科院植物分子生理学重点实验室
作者单位	1.[Yan, Jiapei; Li, Shibai; Kim, Yeon Jeong; Zeng, Qingning; Wang, Lei; Somers, David E.] Ohio State Univ, Mol Genet, Columbus, OH 43210 USA 2.Mem Sloan Kettering Canc Ctr, Mol Biol Program, 1275 York Ave, New York, NY 10021 USA 3.Radziejwoski, Amandine; Somers, David E.] POSTECH, Div Integrat Biosci & Biotechnol, Pohang, South Korea 4.Chinese Acad Sci, Inst Bot, Beijing, Peoples R China 5.RIKEN Ctr Sustainable Resource Sci CSRS, Plant Prote Res Unit, Yokohama, Kanagawa, Japan 6.Max Planck Inst Plant Breeding Res, Prot Mass Spectrometry, Cologne, Germany
推荐引用方式 GB/T 7714	Yan, Jiapei,Li, Shibai,Kim, Yeon Jeong,et al. TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth[J]. EMBO JOURNAL,2021,40(24).
APA	Yan, Jiapei.,Li, Shibai.,Kim, Yeon Jeong.,Zeng, Qingning.,Radziejwoski, Amandine.,...&Somers, David E..(2021).TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth.EMBO JOURNAL,40(24).
MLA	Yan, Jiapei,et al."TOC1 clock protein phosphorylation controls complex formation with NF-YB/C to repress hypocotyl growth".EMBO JOURNAL 40.24(2021).

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