A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus

doi:10.3389/fmicb.2021.741861

	A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus
	Hao, Yuanpeng 1,2; Li, Jingyi 1; Shi, Lei1
	2021
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	12
摘要	Oregano essential oil (OEO), as a natural antimicrobial, has gained increased interest from food researchers and manufacturers. However, a few studies have investigated its possible antibacterial effects against Staphylococcus aureus using the proteomic tool. The present study aimed to explore the antibacterial effect and mechanism of a carvacrol-rich OEO extracted from Origanum vulgare Hot & Spicy on the inactivation of S. aureus. The gas chromatography-mass spectrometry analysis of the OEO allowed the detection of 27 compounds; the major constituent was carvacrol (84.38% of total compounds). The average diameter of the inhibitory zone (DIZ) value was 29.10 mm, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of OEO against S. aureus were 0.125 and 0.25 mg/mL, respectively. The growth curve assay indicated that the OEO prolonged the lag phase of S. aureus. The decrease in cell viability, changes in the integrity of cell membrane, and abnormal cell morphology further reflected the cell damage of S. aureus caused by the OEO. In addition, a label-free proteomic analysis was applied to analyze the regulatory networks of S. aureus in response to 1/2 MIC OEO-treatment stress. Of the 56 differentially expressed proteins (DEPs) identified, 26 were significantly upregulated and 30 downregulated. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the DEPs were mainly involved in pathways of ribosomes; valine, leucine, and isoleucine biosynthesis; and phenylalanine, tyrosine, and tryptophan biosynthesis, which suggested that the growth inhibition of S. aureus might be due to the disordered effect of the OEO on protein synthesis and amino acid metabolism. These findings deepened our understanding regarding S. aureus survival and metabolism responses to the OEO treatment and suggested that the carvacrol-rich OEO could be used in food production environments to effectively control S. aureus.
关键词	carvacrol cell membrane oregano essential oil proteomics Staphylococcus aureus
学科领域	Microbiology
DOI	10.3389/fmicb.2021.741861
收录类别	SCI
语种	英语
WOS关键词	ANTIMICROBIAL ACTIVITY ; THYMOL ; L.
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000720323500001
出版者	FRONTIERS MEDIA SA
文献子类	Article
出版地	LAUSANNE
EISSN	1664-302X
作者邮箱	jingyileesd@126.com ; shilei_67@126.com
作品OA属性	gold, Green Published
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26794
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Hao, Yuanpeng,Li, Jingyi,Shi, Lei. A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus[J]. FRONTIERS IN MICROBIOLOGY,2021,12.
APA	Hao, Yuanpeng,Li, Jingyi,&Shi, Lei.(2021).A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus.FRONTIERS IN MICROBIOLOGY,12.
MLA	Hao, Yuanpeng,et al."A Carvacrol-Rich Essential Oil Extracted From Oregano (Origanum vulgare Hot & Spicy) Exerts Potent Antibacterial Effects Against Staphylococcus aureus".FRONTIERS IN MICROBIOLOGY 12(2021).

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