Genome-Wide Identification and Analyses of Drought/Salt-Responsive Cytochrome P450 Genes in Medicago truncatula

doi:10.3390/ijms22189957

	Genome-Wide Identification and Analyses of Drought/Salt-Responsive Cytochrome P450 Genes in Medicago truncatula
	Xia, Yaying 1,2; Yang, Junfeng 1,2; Ma, Lin ; Yan, Su ; Pang, Yongzhen
	2021
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷号	22 期号:18
摘要	Cytochrome P450 monooxygenases (P450s) catalyze a great number of biochemical reactions and play vital roles in plant growth, development and secondary metabolism. As yet, the genome-scale investigation on P450s is still lacking in the model legume Medicago truncatula. In particular, whether and how many MtP450s are involved in drought and salt stresses for Medicago growth, development and yield remain unclear. In this study, a total of 346 MtP450 genes were identified and classified into 10 clans containing 48 families. Among them, sixty-one MtP450 genes pairs are tandem duplication events and 10 MtP450 genes are segmental duplication events. MtP450 genes within one family exhibit high conservation and specificity in intron-exon structure. Meanwhile, many Mt450 genes displayed tissue-specific expression pattern in various tissues. Specifically, the expression pattern of 204 Mt450 genes under drought/NaCl treatments were analyzed by using the weighted correlation network analysis (WGCNA). Among them, eight genes (CYP72A59v1, CYP74B4, CYP71AU56, CYP81E9, CYP71A31, CYP704G6, CYP76Y14, and CYP78A126), and six genes (CYP83D3, CYP76F70, CYP72A66, CYP76E1, CYP74C12, and CYP94A52) were found to be hub genes under drought/NaCl treatments, respectively. The expression levels of these selected hub genes could be induced, respectively, by drought/NaCl treatments, as validated by qPCR analyses, and most of these genes are involved in the secondary metabolism and fatty acid pathways. The genome-wide identification and co-expression analyses of M. truncatula P450 superfamily genes established a gene atlas for a deep and systematic investigation of P450 genes in M. truncatula, and the selected drought-/salt-responsive genes could be utilized for further functional characterization and molecular breeding for resistance in legume crops.
关键词	cytochrome P450 monooxygenase M truncatula expression profile co-expression analysis salt stress drought stress
学科领域	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
DOI	10.3390/ijms22189957
收录类别	SCI
语种	英语
WOS关键词	GERANIOL 10-HYDROXYLASE ; ARABIDOPSIS-THALIANA ; FATTY-ACIDS ; BIOSYNTHESIS ; STRESS ; EXPRESSION ; FAMILY ; P450 ; CATALYZE ; CLONING
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
WOS记录号	WOS:000700669200001
出版者	MDPI
文献子类	Article
出版地	BASEL
EISSN	1422-0067
资助机构	National Nature Science Foundation of China [U1906201, 31802118] ; Fundamental Research Funds for Central Non-profit Scientific Institution [2021YWF-ZX-05]
作者邮箱	xiayayin0703@126.com ; fyang63@ibcas.ac.cn ; malin@caas.cn ; 17806284739@163.com ; pangyongzhen@caas.cn
作品OA属性	gold, Green Published
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/26825
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xia, Yaying,Yang, Junfeng,Ma, Lin,et al. Genome-Wide Identification and Analyses of Drought/Salt-Responsive Cytochrome P450 Genes in Medicago truncatula[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(18).
APA	Xia, Yaying,Yang, Junfeng,Ma, Lin,Yan, Su,&Pang, Yongzhen.(2021).Genome-Wide Identification and Analyses of Drought/Salt-Responsive Cytochrome P450 Genes in Medicago truncatula.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(18).
MLA	Xia, Yaying,et al."Genome-Wide Identification and Analyses of Drought/Salt-Responsive Cytochrome P450 Genes in Medicago truncatula".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.18(2021).

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