Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition

doi:10.1111/jipb.12275

IB-CAS > 中科院光生物学重点实验室

	Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition
	Yang, Haomeng ; Liao, Libing 1; Bo, Tingting 1; Zhao, Lei 1; Sun, Xuwu ; Lu, Xuefeng 3; Norling, Birgitta 2; Huang, Fang
	2014
发表期刊	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
ISSN	1672-9072
卷号	56 期号:12 页码:1136-1150
摘要	Cyanobacteria are ancient photosynthetic prokaryotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem II (PSII) in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is limited. We have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis sp. PCC 6803. Here, we report that Slr0151 is involved in PSII repair of the organism. We generated a mutant strain (Dslr0151) lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSII activity in Synechocystis. Moreover, the mutant exhibited increased photoinhibition due to impairment of PSII repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSII repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSII in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together, these results indicate that Slr0151 plays an important role in regulating PSII repair in the organism under high-light stress condition.
关键词	Cyanobacteria membrane protein photosystem II repair Slr0151 Synechocystis
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1111/jipb.12275
收录类别	SCI
语种	英语
WOS关键词	SP STRAIN PCC-6803 ; ENVIRONMENTAL-STRESS ; PLASMA-MEMBRANES ; GENE-EXPRESSION ; D1 PROTEIN ; COMPLEX ; CYANOBACTERIA ; PHOTOINHIBITION ; ACCUMULATION ; PROTEOMICS
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000345845200004
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1744-7909
资助机构	National Natural Science Foundation of China [31000018, 30770037] ; Ministry of Science and Technology of China [2009CB220000] ; Chinese Academy of Sciences ; NTU, Singapore [M408030]
作者邮箱	fhuang@ibcas.ac.cn
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27050
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Yang, Haomeng,Liao, Libing,Bo, Tingting,et al. Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2014,56(12):1136-1150.
APA	Yang, Haomeng.,Liao, Libing.,Bo, Tingting.,Zhao, Lei.,Sun, Xuwu.,...&Huang, Fang.(2014).Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,56(12),1136-1150.
MLA	Yang, Haomeng,et al."Slr0151 in Synechocystis sp PCC 6803 is required for efficient repair of photosystem II under high-light condition".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 56.12(2014):1136-1150.

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