Overexpression of stress-inducible OsBURP16, the beta subunit of polygalacturonase 1, decreases pectin content and cell adhesion and increases abiotic stress sensitivity in rice

doi:10.1111/pce.12223

	Overexpression of stress-inducible OsBURP16, the beta subunit of polygalacturonase 1, decreases pectin content and cell adhesion and increases abiotic stress sensitivity in rice
	Liu, Huanhuan 1; Ma, Yan 1; Chen, Na 1; Guo, Siyi 1; Liu, Huili ; Guo, Xiaoyu 1; Chong, Kang ; Xu, Yunyuan
	2014
发表期刊	PLANT CELL AND ENVIRONMENT
ISSN	0140-7791
卷号	37 期号:5 页码:1144-1158
摘要	Polygalacturonase (PG), one of the hydrolases responsible for cell wall pectin degradation, is involved in organ consenescence and biotic stress in plants. PG1 is composed of a catalytic subunit, PG2, and a non-catalytic PG1 beta subunit. OsBURP16 belongs to the PG1 beta-like subfamily of BURP-family genes and encodes one putative PG1 beta subunit precursor in rice (Oryza sativa L.). Transcription of OsBURP16 is induced by cold, salinity and drought stresses, as well as by abscisic acid (ABA) treatment. Analysis of plant survival rates, relative ion leakage rates, accumulation levels of H2O2 and water loss rates of leaves showed that overexpression of OsBURP16 enhanced sensitivity to cold, salinity and drought stresses compared with controls. Young leaves of Ubi::OsBURP16 transgenic plants showed reduced cell adhesion and increased cuticular transpiration rate. Mechanical strength measurement of Ubi::OsBURP16 plants showed that reduced force was required to break leaves as compared with wild type. Transgenic rice showed enhanced PG activity and reduced pectin content. All these results suggested that overexpression of OsBURP16 caused pectin degradation and affected cell wall integrity as well as transpiration rate, which decreased tolerance to abiotic stresses.
关键词	abiotic stress cell adhesion polygalacturonase pectin
学科领域	Plant Sciences
DOI	10.1111/pce.12223
收录类别	SCI
语种	英语
WOS关键词	TREHALOSE-6-PHOSPHATE PHOSPHATASE ; STOMATAL CLOSURE ; COLD TOLERANCE ; SALT STRESS ; GENE ; EXPRESSION ; ARABIDOPSIS ; DROUGHT ; LEAF ; ISOENZYME-1
WOS研究方向	Plant Sciences
WOS记录号	WOS:000333705200010
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1365-3040
资助机构	Chinese Ministry of Agriculture [2014ZX08009-003-002] ; Chinese Academy of Sciences (CAS) [KSCX2-EW-J-1] ; National Natural Science Foundation of China [31070695]
作者邮箱	xuyy@ibcas.ac.cn
作品OA属性	Green Published, hybrid
引用统计	被引频次：98[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27051
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Huanhuan,Ma, Yan,Chen, Na,et al. Overexpression of stress-inducible OsBURP16, the beta subunit of polygalacturonase 1, decreases pectin content and cell adhesion and increases abiotic stress sensitivity in rice[J]. PLANT CELL AND ENVIRONMENT,2014,37(5):1144-1158.
APA	Liu, Huanhuan.,Ma, Yan.,Chen, Na.,Guo, Siyi.,Liu, Huili.,...&Xu, Yunyuan.(2014).Overexpression of stress-inducible OsBURP16, the beta subunit of polygalacturonase 1, decreases pectin content and cell adhesion and increases abiotic stress sensitivity in rice.PLANT CELL AND ENVIRONMENT,37(5),1144-1158.
MLA	Liu, Huanhuan,et al."Overexpression of stress-inducible OsBURP16, the beta subunit of polygalacturonase 1, decreases pectin content and cell adhesion and increases abiotic stress sensitivity in rice".PLANT CELL AND ENVIRONMENT 37.5(2014):1144-1158.

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