Cold acclimation-induced freezing tolerance of Medicago truncatula seedlings is negatively regulated by ethylene

doi:10.1111/ppl.12161

	Cold acclimation-induced freezing tolerance of Medicago truncatula seedlings is negatively regulated by ethylene
	Zhao, Mingui ; Liu, Wenjing 1; Xia, Xiuzhi 1; Wang, Tianzuo ; Zhang, Wen-Hao
	2014
发表期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
卷号	152 期号:1 页码:115-129
摘要	To evaluate the role of ethylene in cold acclimation and cold stress, freezing tolerance and characteristics associated with cold acclimation were investigated using legume model plant Medicago truncatula Gaertn Jemalong A17. There was a rapid suppression of ethylene production during cold acclimation in A17 plants. Ethylene level was negatively correlated with freezing tolerance as inhibition of ethylene biosynthesis by inhibitors of ethylene biosynthesis enhanced freezing tolerance, while exogenous application of ethylene reduced cold acclimation-induced freezing tolerance. The involvement of ethylene signaling in modulation of freezing tolerance and cold acclimation was further studied using ethylene-insensitive mutant sickle skl. Although skl mutant was more tolerant to freezing than its wild-type counterpart A17 plants, cold acclimation enhanced freezing tolerance in 17 plants, but not in skl mutant. Expression of several ethylene response genes including EIN3, EIN3/EIL and ERFs was suppressed in skl mutant compared to A17 plants under non-cold-acclimated conditions. Cold acclimation downregulated expression of EIN3, EIN3/EIL and ERFs in A17 plants, while expression patterns of these genes were relatively constant in skl mutant during cold acclimation. Cold acclimation-induced increases in transcription of MtCBFs and MtCAS15 were suppressed in skl mutant compared with A17 plants. These results suggest that MtSKL1 is required for perception of the change of ethylene level in M. truncatula plants for the full development of the cold acclimation response by suppressing expression of MtEIN3 and MtEIN3/EIL1, which in turn downregulates expression of MtERF s, leading to the enhanced tolerance of M. truncatula to freezing by upregulating MtCBFs and MtCAS15.
学科领域	Plant Sciences
DOI	10.1111/ppl.12161
收录类别	SCI
语种	英语
WOS关键词	TRANSCRIPTION FACTOR ; OXIDATIVE STRESS ; NITRIC-OXIDE ; FACTOR GENE ; ARABIDOPSIS ; EXPRESSION ; EIN2 ; RESPONSES ; SALT ; CBF
WOS研究方向	Plant Sciences
WOS记录号	WOS:000340682100009
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1399-3054
资助机构	Natural Science Foundation of China [90817011, 31370300] ; State Key Laboratory of Vegetation and Environmental Change [80006F2068]
作者邮箱	whzhang@ibcas.ac.cn
引用统计	被引频次：88[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27053
专题	植被与环境变化国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Beijing Inst Life Sci, Res Network Global Change Biol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Mingui,Liu, Wenjing,Xia, Xiuzhi,et al. Cold acclimation-induced freezing tolerance of Medicago truncatula seedlings is negatively regulated by ethylene[J]. PHYSIOLOGIA PLANTARUM,2014,152(1):115-129.
APA	Zhao, Mingui,Liu, Wenjing,Xia, Xiuzhi,Wang, Tianzuo,&Zhang, Wen-Hao.(2014).Cold acclimation-induced freezing tolerance of Medicago truncatula seedlings is negatively regulated by ethylene.PHYSIOLOGIA PLANTARUM,152(1),115-129.
MLA	Zhao, Mingui,et al."Cold acclimation-induced freezing tolerance of Medicago truncatula seedlings is negatively regulated by ethylene".PHYSIOLOGIA PLANTARUM 152.1(2014):115-129.

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