Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits

doi:10.1007/s11434-014-0141-9

	Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits
	Zhao, Xue 1; Han, Feng 2; Shen, Shihua
	2014
发表期刊	CHINESE SCIENCE BULLETIN
ISSN	1001-6538
卷号	59 期号:13 页码:1392-1404
摘要	The chloroplast-to-chromoplast difference is a highly regulated process. Tomato (Solanum lycopersicum) high-pigment mutant hp-1 shows an enhanced accumulation of carotenoids coupled with an increase in the volume and number of plastids. However, how chromoplasts of hp-1 acquire their specific metabolic characteristics is still unclear. A comparison of proteome profiles from plastids at the mature green, breaker, and red stages of tomato fruits showed 45 differentially expressed proteins. These identified proteins fell into six different functional categories. Our results showed that most of the proteins related to the Calvin cycle increased transitorily only at the early breaker stage, and remained unchanged at the early red stage. We found 18 proteins that were differentially expressed between the wild type and hp-1. The abundance of GCPE in chromoplasts of hp-1 was higher than that in the wild type. Meanwhile, we found that heat shock proteins were only present specifically in the mature green stage chromoplasts of hp-1 but not in the wild type. This suggested that GCPE and heat shock proteins might play important roles in the accumulation of high carotenoids in hp-1. Overall, our results could be helpful for understanding chloroplast-to-chromoplast differentiation and the function of hp-1.
关键词	Plastid differentiation High pigment-1 Proteomics Tomato fruit ripening Two-dimensional electrophoresis (2-DE)
学科领域	Multidisciplinary Sciences
DOI	10.1007/s11434-014-0141-9
收录类别	SCI
语种	英语
WOS关键词	CAROTENOID BIOSYNTHESIS ; CHROMOPLAST TRANSITION ; FTSH PROTEASE ; CHLOROPLAST ; SYNTHASE ; PROTEINS ; PATHWAYS ; REVEALS ; PLANTS ; STRESS
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000333613100007
出版者	SCIENCE PRESS
文献子类	Article
出版地	BEIJING
EISSN	1861-9541
资助机构	Key Directional Project from the Chinese Academy of Sciences [KZCX2-YW-359-2]
作者邮箱	shshen@ibcas.ac.cn
引用统计
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27075
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xue,Han, Feng,Shen, Shihua. Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits[J]. CHINESE SCIENCE BULLETIN,2014,59(13):1392-1404.
APA	Zhao, Xue,Han, Feng,&Shen, Shihua.(2014).Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits.CHINESE SCIENCE BULLETIN,59(13),1392-1404.
MLA	Zhao, Xue,et al."Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits".CHINESE SCIENCE BULLETIN 59.13(2014):1392-1404.

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