Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera

doi:10.1016/j.gene.2013.11.047

	Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera
	Sun, Jingwen 1; Peng, Xianjun ; Fan, Weihong ; Tang, Mingjuan ; Liu, Jie ; Shen, Shihua
	2014
发表期刊	GENE
ISSN	0378-1119
卷号	535 期号:2 页码:140-149
摘要	The dehydration-responsive element binding proteins (DREBs) are important transcription factors in the regulation of plant responses to abiotic stresses. In this study, BpDREB2, an AP2/DREB-type transcription factor gene, was cloned from a woody plant, Broussonetia papyrifera by RACE-PCR Sequence analyses revealed that BpDREB2 protein has three characteristic domains, including an AP2/EREBP, a nuclear localization signal and an acidic activation domain. Yeast one-hybrid assays showed that BpDREB2 protein specifically binds to the ORE sequence and activates the expression of reporter genes in yeast. These results suggested that BpDREB2 protein could function as a transcription factor of DREB family. The expression of BpDREB2 gene was remarkably induced by dehydration and high-salt treatments, but no significant change was observed under ABA or low-temperature conditions. Importantly, transgenic expression of BpDREB2 gene in Arabidopsis significantly enhanced its tolerance to salt and freezing without causing growth retardation. Taken together, these results suggested that BpDREB2 is a novel member of the AP2/EREBP trans-acting factor family which could enhance salt stress tolerance of plants and has the potential application in the improvement of crops and economical tree species. (C) 2013 Elsevier B.V. All rights reserved.
关键词	AP2/EREBP-domain Broussonetia papyrifera DREB Salt and cold tolerance Woody plant
学科领域	Genetics & Heredity
DOI	10.1016/j.gene.2013.11.047
收录类别	SCI
语种	英语
WOS关键词	BINDING TRANSCRIPTION FACTOR ; FREEZING TOLERANCE ; ARABIDOPSIS ; COLD ; DEHYDRATION ; PROTEINS ; ELEMENT ; EXPRESSION ; L. ; STRESSES
WOS研究方向	Genetics & Heredity
WOS记录号	WOS:000331672200009
出版者	ELSEVIER
文献子类	Article
出版地	AMSTERDAM
EISSN	1879-0038
资助机构	Chinese Academy of Sciences [KSCX2-YW-G-359-2]
作者邮箱	shshen@ibcas.ac.cn
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27077
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Crop Nutr & Fertilizat, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Sun, Jingwen,Peng, Xianjun,Fan, Weihong,et al. Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera[J]. GENE,2014,535(2):140-149.
APA	Sun, Jingwen,Peng, Xianjun,Fan, Weihong,Tang, Mingjuan,Liu, Jie,&Shen, Shihua.(2014).Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera.GENE,535(2),140-149.
MLA	Sun, Jingwen,et al."Functional analysis of BpDREB2 gene involved in salt and drought response from a woody plant Broussonetia papyrifera".GENE 535.2(2014):140-149.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
24315817.pdf（2124KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文