ARP2/3 complex-mediated actin dynamics is required for hydrogen peroxide-induced stomatal closure in Arabidopsis

doi:10.1111/pce.12259

	ARP2/3 complex-mediated actin dynamics is required for hydrogen peroxide-induced stomatal closure in Arabidopsis
	Li, Xin ; Li, Jian-Hua ; Wang, Wei ; Chen, Nai-Zhi ; Ma, Tong-Suo ; Xi, Ya-Nan ; Zhang, Xiao-Lu ; Lin, Hai-Fei ; Bai, Yang ; Huang, Shan-Jin ; Chen, Yu-Ling
	2014
发表期刊	PLANT CELL AND ENVIRONMENT
ISSN	0140-7791
卷号	37 期号:7 页码:1548-1560
摘要	Multiple cellular events like dynamic actin reorganization and hydrogen peroxide (H2O2) production were demonstrated to be involved in abscisic acid (ABA)-induced stomatal closure. However, the relationship between them as well as the underlying mechanisms remains poorly understood. Here, we showed that H2O2 generation is indispensable for ABA induction of actin reorganization in guard cells of Arabidopsis that requires the presence of ARP2/3 complex. H2O2-induced stomatal closure was delayed in the mutants of arpc4 and arpc5, and the rate of actin reorganization was slowed down in arpc4 and arpc5 in response to H2O2, suggesting that ARP2/3-mediated actin nucleation is required for H2O2-induced actin cytoskeleton remodelling. Furthermore, the expression of H2O2 biosynthetic related gene AtrbohD and the accumulation of H2O2 was delayed in response to ABA in arpc4 and arpc5, demonstrating that misregulated actin dynamics affects H2O2 production upon ABA treatment. These results support a possible causal relation between the production of H2O2 and actin dynamics in ABA-mediated guard cell signalling: ABA triggers H2O2 generation that causes the reorganization of the actin cytoskeleton partially mediated by ARP2/3 complex, and ARP2/3 complex-mediated actin dynamics may feedback regulate H2O2 production.
关键词	ABA ARPC4 ARPC5 guard cells
学科领域	Plant Sciences
DOI	10.1111/pce.12259
收录类别	SCI
语种	英语
WOS关键词	OXYGEN SPECIES PRODUCTION ; CELL SIGNAL-TRANSDUCTION ; HETEROTRIMERIC G-PROTEIN ; GUARD-CELLS ; ABSCISIC-ACID ; VICIA-FABA ; PLASMA-MEMBRANE ; NADPH-OXIDASE ; NITRIC-OXIDE ; CA2+ CHANNELS
WOS研究方向	Plant Sciences
WOS记录号	WOS:000337544200006
出版者	WILEY-BLACKWELL
文献子类	Article
出版地	HOBOKEN
EISSN	1365-3040
资助机构	National Science Foundation of China [30971506, 30970266, 30670173, 31125004] ; Excellent Youth Foundation of Hebei Scientific Committee [C2010000411] ; program for New Century Excellent Talents in University [NCET-10-0128]
作者邮箱	sjhuang@ibcas.ac.cn ; yulingchen@mail.hebtu.edu.cn
作品OA属性	Bronze
引用统计	被引频次：37[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27084
专题	中科院植物分子生理学重点实验室
作者单位	1.[Li, Xin 2.Li, Jian-Hua 3.Wang, Wei 4.Xi, Ya-Nan 5.Zhang, Xiao-Lu 6.Lin, Hai-Fei 7.Bai, Yang 8.Hebei Normal Univ, Coll Life Sci, Hebei Key Lab Mol & Cellular Biol, Shijiazhuang 050024, Peoples R China 9.Chen, Nai-Zhi 10.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 11.Hebei Univ Econ & Business, Coll Biol Sci & Engn, Shijiazhuang 050061, Peoples R China
推荐引用方式 GB/T 7714	Li, Xin,Li, Jian-Hua,Wang, Wei,et al. ARP2/3 complex-mediated actin dynamics is required for hydrogen peroxide-induced stomatal closure in Arabidopsis[J]. PLANT CELL AND ENVIRONMENT,2014,37(7):1548-1560.
APA	Li, Xin.,Li, Jian-Hua.,Wang, Wei.,Chen, Nai-Zhi.,Ma, Tong-Suo.,...&Chen, Yu-Ling.(2014).ARP2/3 complex-mediated actin dynamics is required for hydrogen peroxide-induced stomatal closure in Arabidopsis.PLANT CELL AND ENVIRONMENT,37(7),1548-1560.
MLA	Li, Xin,et al."ARP2/3 complex-mediated actin dynamics is required for hydrogen peroxide-induced stomatal closure in Arabidopsis".PLANT CELL AND ENVIRONMENT 37.7(2014):1548-1560.

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