Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis

doi:10.1093/pcp/pcu087

	Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis
	Yu, Pengli ; Yuan, Jinhong ; Deng, Xin1,2 ; Ma, Mi ; Zhang, Haiyan
	2014
发表期刊	PLANT AND CELL PHYSIOLOGY
ISSN	0032-0781
卷号	55 期号:9 页码:1568-1581
摘要	Copper (Cu) is an important environmental pollutant that exerts harmful effects on all living organisms when in excess. In an effort to remove this toxin in situ, a bacterial Cu-binding protein gene CusF was engineered to target CusF for secretion to the cell wall and vacuoles and for accumulation in the cytoplasm. Analysis of transgenic Arabidopsis plants showed that CusF was functionally active and that plants expressing cell wall-(CusF(cw) transgenic lines) or vacuole-targeted CusF (CusF(vac) transgenic lines) were more resistant to Cu excess than untransformed plants and plants with cytoplasmic CusF (CusF(cyto) transgenic lines). Under short-term (48 h) exposure to Cu excess, CusFcw transgenic lines showed up to 2-fold increased Cu accumulation in roots compared with the untransformed plants; however, CusFcyto lines and the wild-type plants had similar Cu concentrations in both roots and shoots. Under long-term (40 d) exposure to Cu excess, all transgenic lines accumulated more Cu (up to 3-fold) in roots than the untransformed plants, whereas only CusFcyto lines showed a marked increase (similar to 3-fold of the wild-type plants) of Cu accumulation in shoots. In addition, expression of CusF in the cytosol dramatically enhanced Cu transport from roots to shoots when compared with plants with secretory pathway-targeted CusF. Our results demonstrate the feasibility of Cu tolerance and accumulation by engineering Cu-binding proteins targetable to subcellular compartments and provide new insights into the multifaceted mechanisms of Cu partitioning between roots and shoots.
关键词	Arabidopsis Cu accumulation CusF Phytoremediation Subcellular targeting Translocation factor
学科领域	Plant Sciences ; Cell Biology
DOI	10.1093/pcp/pcu087
收录类别	SCI
语种	英语
WOS关键词	ESCHERICHIA-COLI ; COPPER TRANSPORT ; ENDOPLASMIC-RETICULUM ; IRON HOMEOSTASIS ; CELL-WALL ; PH ; METALLOTHIONEINS ; COMPARTMENTATION ; TOLERANCE ; CADMIUM
WOS研究方向	Plant Sciences ; Cell Biology
WOS记录号	WOS:000342978300005
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1471-9053
资助机构	National Natural Science Foundation of China [31270313, 90817010]
作者邮箱	hyz@ibcas.ac.cn
作品OA属性	Bronze
引用统计	被引频次：17[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27087
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 3.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA
推荐引用方式 GB/T 7714	Yu, Pengli,Yuan, Jinhong,Deng, Xin,et al. Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis[J]. PLANT AND CELL PHYSIOLOGY,2014,55(9):1568-1581.
APA	Yu, Pengli,Yuan, Jinhong,Deng, Xin,Ma, Mi,&Zhang, Haiyan.(2014).Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis.PLANT AND CELL PHYSIOLOGY,55(9),1568-1581.
MLA	Yu, Pengli,et al."Subcellular Targeting of Bacterial CusF Enhances Cu Accumulation and Alters Root to Shoot Cu Translocation in Arabidopsis".PLANT AND CELL PHYSIOLOGY 55.9(2014):1568-1581.

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