OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4

doi:10.1104/pp.114.235564

	OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4
	Liu, Huanhuan ; Guo, Siyi ; Xu, Yunyuan ; Li, Chunhua ; Zhang, Zeyong ; Zhang, Dajian ; Xu, Shujuan ; Zhang, Cui; Chong, Kang 1
	2014
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	165 期号:1 页码:160-174
摘要	Inflorescence and spikelet development determine grain yields in cereals. Although multiple genes are known to be involved in the regulation of floral organogenesis, the underlying molecular network remains unclear in cereals. Here, we report that the rice (Oryza sativa) microRNA396d (OsmiR396d) and its Os Growth Regulating Factor (OsGRF) targets, together with Os Growth Regulating Factor-Interacting Factor1 (OsGIF1), are involved in the regulation of floral organ development through the rice JMJD2 family jmjC gene 706 (OsJMJ706) and crinkly4 receptor-like kinase (OsCR4). Transgenic knockdown lines of OsGRF6, a predicted target of OsmiR396d, and overexpression lines of OsmiR396d showed similar defects in floral organ development, including open husks, long sterile lemmas, and altered floral organ morphology. These defects were almost completely rescued by overexpression of OsGRF6. OsGRF6 and its ortholog OsGRF10 were the most highly expressed OsGRF family members in young inflorescences, and the grf6/grf10 double mutant displayed abnormal florets. OsGRF6/OsGRF10 localized to the nucleus, and electrophoretic mobility shift assays revealed that both OsGRF6 and OsGRF10 bind the GA response element in the promoters of OsJMJ706 and OsCR4, which were reported to participate in the regulation of floral organ development. In addition, OsGRF6 and OsGRF10 could transactivate OsJMJ706 and OsCR4, an activity that was enhanced in the presence of OsGIF1, which can bind both OsGRF6 and OsGRF10. Together, our results suggest that OsmiR396d regulates the expression of OsGRF genes, which function with OsGIF1 in floret development through targeting of JMJ706 and OsCR4. This work thus reveals a microRNA-mediated regulation module for controlling spikelet development in rice.
学科领域	Plant Sciences
DOI	10.1104/pp.114.235564
收录类别	SCI
语种	英语
WOS关键词	MADS-BOX GENE ; ARABIDOPSIS-THALIANA ; CELL-PROLIFERATION ; ECTOPIC EXPRESSION ; ORGAN IDENTITY ; MERISTEM FATE ; TRANSCRIPTION FACTORS ; SPIKELET DEVELOPMENT ; FLOWER DEVELOPMENT ; HOMEOTIC GENE
WOS研究方向	Plant Sciences
WOS记录号	WOS:000335906300013
出版者	AMER SOC PLANT BIOLOGISTS
文献子类	Article
出版地	ROCKVILLE
EISSN	1532-2548
资助机构	National Natural Sciences Foundation of China [31270331, 31121065]
作者邮箱	chongk@ibcas.ac.cn
作品OA属性	Green Published, Bronze
引用统计	被引频次：155[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27089
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Natl Ctr Plant Gene Res Beijing, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Liu, Huanhuan,Guo, Siyi,Xu, Yunyuan,et al. OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4[J]. PLANT PHYSIOLOGY,2014,165(1):160-174.
APA	Liu, Huanhuan.,Guo, Siyi.,Xu, Yunyuan.,Li, Chunhua.,Zhang, Zeyong.,...&Chong, Kang.(2014).OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4.PLANT PHYSIOLOGY,165(1),160-174.
MLA	Liu, Huanhuan,et al."OsmiR396d-Regulated OsGRFs Function in Floral Organogenesis in Rice through Binding to Their Targets OsJMJ706 and OsCR4".PLANT PHYSIOLOGY 165.1(2014):160-174.

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