Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars

doi:10.1007/s00299-014-1642-3

	Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars
	Fang, Linchuan 1; Hou, Yanlin 2; Wang, Lijun 3; Xin, Haiping 3; Wang, Nian 4; Li, Shaohua3
	2014
发表期刊	PLANT CELL REPORTS
ISSN	0721-7714
卷号	33 期号:10 页码:1629-1640
摘要	High and low resveratrol (Res) contents in two cultivars are correlated with the expression abundance of Myb14 , which could directly activate transcriptional expression of stilbene synthase gene ( STS ). Resveratrol (3,5,4'-trihydroxystilbene) is one of the natural polyphenols produced by secondary metabolism in some plants. Stilbene synthase (STS) is the key enzyme for the final step of precursor formation of resveratrol (Res) in grapevines. In this study, we found that Res contents in ripe berry skin were completely different in two grape cultivars, namely, 'Z168' (Vitis monticola x Vitis riparia) with high-Res and 'Jingzaojing' (Vitis vinifera) with low-Res. Moreover, the level of expression of STS gene was higher in the ripe berry skin of 'Z168' than in that of 'Jingzaojing'. To further investigate the underlying mechanisms, we conducted a co-expression analysis through transcriptomic data. We confirmed that Myb14, an R2R3 Myb transcription factor, is the direct regulator of STS by binding to Box-L5 motif. Moreover, the expression pattern of Myb14 is associated with the variation of Res content. To test this prediction, we conducted a number of experiments in vivo and in vitro. The expression patterns of Myb14 and STS in grapevine leaves were identical under a series of stimulus. Myb14 showed higher expression in the ripe berry skin of 'Z168' than in that of 'Jingzaojing'. Yeast one-hybrid assay indicated that grapevine Myb14 could interact with the promoter of STS in vitro, and the transient overexpression of Myb14 promoted the expression of STS. Furthermore, co-expressing 35S::Myb14 in transgenic Arabidopsis could activate GUS expression promoted by STS promoter. Thus, Myb14 is the direct activator of STS, and its expression pattern is associated with Res content variation in grapes.
关键词	Resveratrol Stilbene synthases STS Myb14 Grapevine
学科领域	Plant Sciences
DOI	10.1007/s00299-014-1642-3
收录类别	SCI
语种	英语
WOS关键词	PHENYLALANINE AMMONIA-LYASE ; STILBENE SYNTHASE ; GENE-EXPRESSION ; VITIS-VINIFERA ; CABERNET-SAUVIGNON ; MOLECULAR ANALYSIS ; CHALCONE SYNTHASE ; UV-C ; BIOSYNTHESIS ; TOLERANCE
WOS研究方向	Plant Sciences
WOS记录号	WOS:000342193100003
出版者	SPRINGER
文献子类	Article
出版地	NEW YORK
EISSN	1432-203X
资助机构	National Natural Science Foundation of China (NSFC) [31171931] ; National Natural Science Foundation of Hubei Province [2011CDB409]
作者邮箱	fanglinchuan@gmail.com ; ylhou@genetics.ac.cn ; ljwang@ibcas.ac.cn ; xinhaiping215@hotmail.com ; nian.wang1982@gmail.com ; shhli@wbgcas.cn
引用统计	被引频次：33[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27092
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Lab Plant Resources, Beijing, Peoples R China 5.Chinese Acad Agr Sci, Oil Crop Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr, Wuhan 430062, Peoples R China
推荐引用方式 GB/T 7714	Fang, Linchuan,Hou, Yanlin,Wang, Lijun,et al. Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars[J]. PLANT CELL REPORTS,2014,33(10):1629-1640.
APA	Fang, Linchuan,Hou, Yanlin,Wang, Lijun,Xin, Haiping,Wang, Nian,&Li, Shaohua.(2014).Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars.PLANT CELL REPORTS,33(10),1629-1640.
MLA	Fang, Linchuan,et al."Myb14, a direct activator of STS, is associated with resveratrol content variation in berry skin in two grape cultivars".PLANT CELL REPORTS 33.10(2014):1629-1640.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Fang-2014-Myb14, a d（778KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文