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| Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis | |
| Hao, Huaiqing; Fan, Lusheng; Chen, Tong; Li, Ruili1,2; Li, Xiaojuan1,2; He, Qihua3; Botella, Miguel A.; Lin, Jinxing1,2 | |
| 2014 | |
| 发表期刊 | PLANT CELL
 |
| ISSN | 1040-4651 |
| 卷号 | 26期号:4页码:1729-1745 |
| 摘要 | Arabidopsis thaliana respiratory burst oxidase homolog D (RbohD) functions as an essential regulator of reactive oxygen species (ROS). However, our understanding of the regulation of RbohD remains limited. By variable-angle total internal reflection fluorescence microscopy, we demonstrate that green fluorescent protein (GFP)-RbohD organizes into dynamic spots at the plasma membrane. These RbohD spots have heterogeneous diffusion coefficients and oligomerization states, as measured by photobleaching techniques. Stimulation with ionomycin and calyculin A, which activate the ROS-producing enzymatic activity of RbohD, increases the diffusion and oligomerization of RbohD. Abscisic acid and flg22 treatments also increase the diffusion coefficient and clustering of GFP-RbohD. Single-particle analysis in clathrin heavy chain2 mutants and a Flotillin1 artificial microRNA line demonstrated that clathrin-and microdomain-dependent endocytic pathways cooperatively regulate RbohD dynamics. Under salt stress, GFP-RbohD assembles into clusters and then internalizes into the cytoplasm. Dual-color fluorescence cross-correlation spectroscopy analysis further showed that salt stress stimulates RbohD endocytosis via membrane microdomains. We demonstrate that microdomain-associated RbohD spots diffuse at the membrane with high heterogeneity, and these dynamics closely relate to RbohD activity. Our results provide insight into the regulation of RbohD activity by clustering and endocytosis, which facilitate the activation of redox signaling pathways. |
| 学科领域 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| DOI | 10.1105/tpc.113.122358 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | RESPIRATORY BURST OXIDASE ; REFLECTION FLUORESCENCE MICROSCOPY ; DETERGENT-RESISTANT MEMBRANES ; POSITIVE FEEDBACK-REGULATION ; SINGLE-MOLECULE DIFFUSION ; REVEALS MULTIPLE-MODES ; LIPID RAFTS ; PROTEIN ; ENDOCYTOSIS ; ATRBOHD |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| WOS记录号 | WOS:000337351300026 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 文献子类 | Article |
| 出版地 | ROCKVILLE |
| EISSN | 1532-298X |
| 资助机构 | National Basic Research Program of China (973 Program) [2011CB944600, 2011CB809103] ; National Natural Science Foundation of China [31121065, 31170163, 31270224] ; Program of Introducing Talents of Discipline to Universities (111 project) [B13007] ; Program for Changjiang Scholars and Innovative Research Team in University [IRT 13047] |
| 作者邮箱 | linjx@ibcas.ac.cn |
| 作品OA属性 | Green Published, Bronze |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/27099 |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China 4.Peking Univ, Hlth Sci Ctr, Beijing 100191, Peoples R China 5.Botella, Miguel A.] Univ Malaga, Dept Biol Celular Genet & Fisiol, E-29071 Malaga, Spain |
| 推荐引用方式 GB/T 7714 | Hao, Huaiqing,Fan, Lusheng,Chen, Tong,et al. Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis[J]. PLANT CELL,2014,26(4):1729-1745. |
| APA | Hao, Huaiqing.,Fan, Lusheng.,Chen, Tong.,Li, Ruili.,Li, Xiaojuan.,...&Lin, Jinxing.(2014).Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis.PLANT CELL,26(4),1729-1745. |
| MLA | Hao, Huaiqing,et al."Clathrin and Membrane Microdomains Cooperatively Regulate RbohD Dynamics and Activity in Arabidopsis".PLANT CELL 26.4(2014):1729-1745. |
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| Hao-2014-Clathrin an（2848KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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