Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing

doi:10.1073/pnas.1316183111

IB-CAS > 中科院光生物学重点实验室

	Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing
	Zhang, Fan 1; Tang, Weijiang ; Hedtke, Boris 2; Zhong, Linlin 1; Liu, Lin ; Peng, Lianwei ; Lu, Congming 3; Grimm, Bernhard 2; Lin, Rongcheng3
	2014
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	111 期号:5 页码:2023-2028
摘要	RNA editing is a posttranscriptional process that covalently alters the sequence of RNA molecules and plays important biological roles in both animals and land plants. In flowering plants, RNA editing converts specific cytidine residues to uridine in both plastid and mitochondrial transcripts. Previous studies identified pentatricopeptide repeat (PPR) motif-containing proteins as site-specific recognition factors for cytidine targets in RNA sequences. However, the regulatory mechanism underlying RNA editing was largely unknown. Here, we report that protoporphyrinogen IX oxidase 1 (PPO1), an enzyme that catalyzes protoporphyrinogen IX into protoporphyrin IX in the tetrapyrrole biosynthetic pathway, plays an unexpected role in editing multiple sites of plastid RNA transcripts, most of which encode subunits of the NADH dehydrogenase-like complex (NDH), in the reference plant Arabidopsis thaliana. We identified multiple organellar RNA editing factors (MORFs), including MORF2, MORF8, and MORF9, that interact with PPO1. We found that two conserved motifs within the 22-aa region at the N terminus of PPO1 are essential for its interaction with MORFs, its RNA editing function, and subsequently, its effect on NDH activity. However, transgenic plants lacking key domains for the tetrapyrrole biosynthetic activity of PPO1 exhibit normal RNA editing. Furthermore, MORF2 and MORF9 interact with three PPRs or related proteins required for editing of ndhB and ndhD sites. These results reveal that the tetrapyrrole biosynthetic enzyme PPO1 is required for plastid RNA editing, acting as a regulator that promotes the stability of MORF proteins through physical interaction.
关键词	metabolism organelle editosome
学科领域	Multidisciplinary Sciences
DOI	10.1073/pnas.1316183111
收录类别	SCI
语种	英语
WOS关键词	PENTATRICOPEPTIDE REPEAT PROTEINS ; PPR PROTEINS ; ARABIDOPSIS ; CHLOROPLAST ; REVEALS ; SITE ; DISRUPTION ; INTERACTS ; MOTIF
WOS研究方向	Science & Technology - Other Topics
WOS记录号	WOS:000330587600082
出版者	NATL ACAD SCIENCES
文献子类	Article
出版地	WASHINGTON
资助机构	National Natural Science Foundation of China [31170221] ; Chinese Academy of Sciences
作者邮箱	rclin@ibcas.ac.cn
作品OA属性	Bronze, Green Published
引用统计	被引频次：111[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27114
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Humboldt Univ, Inst Biol, Dept Plant Physiol, D-10099 Berlin, Germany 4.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Fan,Tang, Weijiang,Hedtke, Boris,et al. Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2014,111(5):2023-2028.
APA	Zhang, Fan.,Tang, Weijiang.,Hedtke, Boris.,Zhong, Linlin.,Liu, Lin.,...&Lin, Rongcheng.(2014).Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,111(5),2023-2028.
MLA	Zhang, Fan,et al."Tetrapyrrole biosynthetic enzyme protoporphyrinogen IX oxidase 1 is required for plastid RNA editing".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 111.5(2014):2023-2028.

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