Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis

doi:10.1093/jb/mvu028

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	Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis
	Liu, Cheng; Rao, Yan; Zhang, Lei; Yang, Chunhong
	2014
发表期刊	JOURNAL OF BIOCHEMISTRY
ISSN	0021-924X
卷号	156 期号:4 页码:203-210
摘要	The functions of the helix E (W97-F105), an amphiphilic lumenal 310 helix of the major antenna of photosystem II (LHCII), are still unidentified. To elucidate the roles of individual amino acid residue of the helix E, alanine scanning mutagenesis has been performed to mutate every residue of this domain to alanine. The influence of every alanine substitution on the structure and function of LHCII has been investigated biochemically and spectroscopically. The results show that all mutations have little impact on the pigment binding and configuration. However, many mutants presented decreased thermo-or photo-stability compared with the wild type, highlighting the significance of this helix to the stability of LHCII. The most critical residue for stability is W97. The mutant W97A yielded very fragile trimeric pigment protein complexes. The structural analysis revealed that the hydrogen bonding and aromatic interactions between W97, F195, F194 and a water molecule contributed greatly to the stability of LHCII. Moreover, Q103A and F105A have been identified to be able to reinforce the tendency of aggregation in vitro. The structural analysis suggested that the enhancement in aggregation formation for Q103A and F105A might be attributed to the changing hydrophobicity of the region.
关键词	photosynthesis aggregation hydrophobicity light-harvesting chlorophyll a/b complexes of photosystem II photosynthesis stability
学科领域	Biochemistry & Molecular Biology
DOI	10.1093/jb/mvu028
收录类别	SCI
语种	英语
WOS关键词	LIGHT-HARVESTING COMPLEX ; CHLOROPHYLL-A/B-COMPLEX ; PIGMENT-BINDING ; LUMENAL LOOP ; IN-VITRO ; XANTHOPHYLL CYCLE ; CRYSTAL-STRUCTURE ; HIGHER-PLANTS ; PROTEIN ; MECHANISM
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000343324700003
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1756-2651
资助机构	National Basic Research Program of China [2011CBA00904] ; Key Research Program of the Chinese Academy of Sciences [KSZD-EW-Z-018] ; National Natural Science Foundation of China [30800069, 31070212, 31370275]
作者邮箱	yangch@ibcas.ac.cn
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27115
专题	中科院光生物学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Liu, Cheng,Rao, Yan,Zhang, Lei,et al. Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis[J]. JOURNAL OF BIOCHEMISTRY,2014,156(4):203-210.
APA	Liu, Cheng,Rao, Yan,Zhang, Lei,&Yang, Chunhong.(2014).Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis.JOURNAL OF BIOCHEMISTRY,156(4),203-210.
MLA	Liu, Cheng,et al."Identification of the roles of individual amino acid residues of the helix E of the major antenna of photosystem II (LHCII) by alanine scanning mutagenesis".JOURNAL OF BIOCHEMISTRY 156.4(2014):203-210.

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