AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana

doi:10.1093/mp/sst092

	AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana
	Yu, Qing-Bo ; Ma, Qian ; Kong, Meng-Meng ; Zhao, Tuan-Tuan ; Zhang, Xiao-Lei ; Zhou, Que ; Huang, Chao ; Chong, Kang 2; Yang, Zhong-Nan
	2014
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	7 期号:1 页码:206-217
摘要	AtECB1/MRL7 with disulfide reductase activity in vitro can interact with Trx Z and FSD3, two intrinsic subunits of plastid-encoded RNA polymerase (PEP) complex. It may play a role in thioredoxin signaling to regulate plastid gene expression and chloroplast development.Plastid-encoded RNA polymerase (PEP) is closely associated with numerous factors to form PEP complex for plastid gene expression and chloroplast development. However, it is not clear how PEP complex are regulated in chloroplast. Here, one thioredoxin-like fold protein, Arabidopsis early chloroplast biogenesis 1 (AtECB1), an allele of MRL7, was identified to regulate PEP function and chloroplast biogenesis. The knockout lines for AtECB1 displayed albino phenotype and impaired chloroplast development. The transcripts of PEP-dependent plastid genes were barely detected, suggesting that the PEP activity is almost lost in atecb1-1. Although AtECB1 was not identified in PEP complex, a yeast two-hybrid assay and pull-down experiments demonstrated that it can interact with Trx Z and FSD3, two intrinsic subunits of PEP complex, respectively. This indicates that AtECB1 may play a regulatory role for PEP-dependent plastid gene expression through these two subunits. AtECB1 contains a structure in the thioredoxin-like fold domain and lacks the typical C-X-X-C active site motif. Insulin assay demonstrated that AtECB1 harbors disulfide reductase activity in vitro using the purified recombinant AtECB1 protein. This showed that this thioredoxin-like fold protein, AtECB1 also has the thioredoxin activity. AtECB1 may play a role in thioredoxin signaling to regulate plastid gene expression and chloroplast development.
关键词	Arabidopsis Thioredoxin disulfide reductase activity chloroplast transcription chloroplast biogenesis
学科领域	Biochemistry & Molecular Biology ; Plant Sciences
DOI	10.1093/mp/sst092
收录类别	SCI
语种	英语
WOS关键词	RNA-POLYMERASE ; CHROMOSOME COMPLEX ; REDOX REGULATION ; REPEAT PROTEIN ; TRANSCRIPTION ; INTERACTS ; GROWTH ; INTEGRATION ; COMPONENT ; PROTEOMES
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000329254200015
出版者	CELL PRESS
文献子类	Article
出版地	CAMBRIDGE
EISSN	1752-9867
资助机构	Shanghai Municipal Natural Science Foundation [10ZR1421800] ; National Science Foundation of China [31100965] ; National Basic Research Program of China [2009CB118504] ; Young Teachers Training Program of Shanghai Municipal Education Commission [shsf021] ; Leading Academic Discipline Project of the Shanghai Municipal Education Commission [J50401]
作者邮箱	znyang@shnu.edu.cn
作品OA属性	Bronze
引用统计	被引频次：34[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27118
专题	中科院植物分子生理学重点实验室
作者单位	1.Shanghai Normal Univ, Dept Biol, Life & Environm Coll, Shanghai 200234, Peoples R China 2.Chinese Acad Sci, Natl Lab Plant Mol Genet, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Res Ctr Mol & Dev Biol, Key Lab Photosynth & Environm Mol Physiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yu, Qing-Bo,Ma, Qian,Kong, Meng-Meng,et al. AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana[J]. MOLECULAR PLANT,2014,7(1):206-217.
APA	Yu, Qing-Bo.,Ma, Qian.,Kong, Meng-Meng.,Zhao, Tuan-Tuan.,Zhang, Xiao-Lei.,...&Yang, Zhong-Nan.(2014).AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana.MOLECULAR PLANT,7(1),206-217.
MLA	Yu, Qing-Bo,et al."AtECB1/MRL7, a Thioredoxin-Like Fold Protein with Disulfide Reductase Activity, Regulates Chloroplast Gene Expression and Chloroplast Biogenesis in Arabidopsis thaliana".MOLECULAR PLANT 7.1(2014):206-217.

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