Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma

doi:10.1007/s12010-014-1025-9

	Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma
	Hao, He ; Lei, Caiyan 1; Dong, QiuLei 2; Shen, Yalin ; Chi, Jianting ; Ye, Hechun 3; Wang, Hong
	2014
发表期刊	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN	0273-2289
卷号	173 期号:8 页码:2198-2210
摘要	The shikonin derivatives, accumulated in the roots of Arnebia euchroma (Boraginaceae), showed antibacterial, anti-inflammatory, and anti-tumor activities. To explore their possible biosynthesis regulation mechanism, this paper investigated the effects of exogenous methyl jasmonate (MJ) on the biosynthesis of shikonin derivatives in callus cultures of A. euchroma. The main results include: Under MJ treatment, the growth of A. euchroma callus cultures was not inhibited, but the expression level of both the genes involved in the biosynthesis of shikonin derivatives and their precursors and the genes responsible for intracellular localization of shikonin derivatives increased significantly in the Red Strain (shikonin derivatives high-producing strain). The quantitative analysis showed that six out of the seven naphthoquinone compounds under investigation increased their contents in the MJ-treated Red Strain, and in particular, the bioactive component acetylshikonin nearly doubled its content in the MJ-treated Red Strain. In addition, it was also observed that the metabolic profiling of naphthoquinone compounds changed significantly after MJ treatment, and the MJ-treated and MJ-untreated strains clearly formed distinct clusters in the score plot of PLS-DA. Our results provide some new insights into the regulation mechanism of the biosynthesis of shikonin derivatives and a possible way to increase the production of naphthoquinone compounds in A. euchroma callus cultures in the future.
关键词	Arnebia euchroma Callus Shikonin derivatives Methyl jasmonate Gene expression
学科领域	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
DOI	10.1007/s12010-014-1025-9
收录类别	SCI
语种	英语
WOS关键词	LITHOSPERMUM CELL-CULTURES ; SUSPENSION-CULTURES ; GENE-EXPRESSION ; ROOT CULTURES ; CDNA CLONING ; ERYTHRORHIZON ; PROTEIN ; ACID
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000340591800021
出版者	HUMANA PRESS INC
文献子类	Article
出版地	TOTOWA
EISSN	1559-0291
资助机构	National Natural Science Foundation of China [61173098]
作者邮箱	hwang@ucas.ac.cn
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27124
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Henan Agr Univ, Coll Plant Protect, Zhengzhou 450002, Peoples R China 3.Chinese Acad Sci, Inst Automat, Natl Lab Pattern Recognit, Beijing 100190, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Hao, He,Lei, Caiyan,Dong, QiuLei,et al. Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2014,173(8):2198-2210.
APA	Hao, He.,Lei, Caiyan.,Dong, QiuLei.,Shen, Yalin.,Chi, Jianting.,...&Wang, Hong.(2014).Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,173(8),2198-2210.
MLA	Hao, He,et al."Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 173.8(2014):2198-2210.

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