The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis

doi:10.1093/jxb/eru140

	The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis
	Qin, Zhixiang ; Zhang, Xiaoran 1; Zhang, Xiao 1; Xin, Wei ; Li, Jia 2; Hu, Yuxin3
	2014
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
ISSN	0022-0957
卷号	65 期号:9 页码:2521-2531
摘要	Male gametogenesis in angiosperms involves two rounds of mitosis that are essential for the generation of two sperm cells to achieve double fertilization, a distinct event in the sexual reproduction of flowering plants. Precise regulation of mitosis during male gametogenesis is critically important for the establishment of the male germline. However, the molecular mechanisms underlying mitotic division during male gametophyte development have not been characterized fully. Here, we report that the Arabidopsis transcription initiation factor TFIIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis. BRP4 was expressed predominately in developing male gametophytes. Knockdown expression of BRP4 by a native promoter-driven RNA interference construct in Arabidopsis resulted in arrest of the mitotic progression of male gametophytes, leading to a defect in pollen development. Moreover, we showed that the level of expression of a gene encoding a subunit of the origin recognition complex, ORC6, was decreased in BRP4 knockdown plants, and that the ORC6 knockdown transgenic plants phenocopied the male gametophyte defect observed in BRP4 knockdown plants, suggesting that ORC6 acts downstream of BRP4 to mediate male mitotic progression. Taken together, our results reveal that BRP4 plays an important role in the regulation of mitotic cell-cycle progression during male gametogenesis.
关键词	Arabidopsis BRP4 male gametogenesis mitosis ORC6 TFIIB-related protein
学科领域	Plant Sciences
DOI	10.1093/jxb/eru140
收录类别	SCI
语种	英语
WOS关键词	MALE GAMETOPHYTE DEVELOPMENT ; TFIIB-RELATED PROTEIN ; ORIGIN RECOGNITION COMPLEX ; GENE-EXPRESSION ; ORGAN GROWTH ; E3 LIGASES ; POLLEN ; THALIANA ; PLANTS ; DIVISION
WOS研究方向	Plant Sciences
WOS记录号	WOS:000337978800024
出版者	OXFORD UNIV PRESS
文献子类	Article
出版地	OXFORD
EISSN	1460-2431
资助机构	National Natural Science Foundation of China [30800599, 31121065] ; Ministry of Science and Technology of China [2009CB941500]
作者邮箱	huyuxin@ibcas.ac.cn
作品OA属性	Green Published, Green Submitted, hybrid
引用统计	被引频次：14[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27144
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 4.Natl Ctr Plant Gene Res, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Qin, Zhixiang,Zhang, Xiaoran,Zhang, Xiao,et al. The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014,65(9):2521-2531.
APA	Qin, Zhixiang,Zhang, Xiaoran,Zhang, Xiao,Xin, Wei,Li, Jia,&Hu, Yuxin.(2014).The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis.JOURNAL OF EXPERIMENTAL BOTANY,65(9),2521-2531.
MLA	Qin, Zhixiang,et al."The Arabidopsis transcription factor IIB-related protein BRP4 is involved in the regulation of mitotic cell-cycle progression during male gametogenesis".JOURNAL OF EXPERIMENTAL BOTANY 65.9(2014):2521-2531.

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