Chloroplast-targeted Hsp90 plays essential roles in plastid development and embryogenesis in Arabidopsis possibly linking with VIPP1

doi:10.1111/ppl.12083

	Chloroplast-targeted Hsp90 plays essential roles in plastid development and embryogenesis in Arabidopsis possibly linking with VIPP1
	Feng, Juanjuan; Fan, Pengxiang; Jiang, Ping; Lv, Sulian; Chen, Xianyang; Li, Yinxin
	2014
发表期刊	PHYSIOLOGIA PLANTARUM
ISSN	0031-9317
卷号	150 期号:2 页码:292-307
摘要	The Arabidopsis genome contains seven members of Hsp90. Mutations in plastid AtHsp90.5 were reported to cause defects in chloroplast development and embryogenesis. However, the exact function of plastid AtHsp90.5 has not yet been defined. In this study, albino seedlings were found among AtHsp90.5 transformed Arabidopsis, which were revealed to be AtHsp90.5 co-suppressed plants. The accumulation of photosynthetic super-complexes in the albinos was decreased, and expression of genes involved in photosynthesis was significantly down-regulated. AtHsp90.5 T-DNA insertion mutants were embryo-lethal with embryo arrested at the heart stage. Further investigation showed AtHsp90.5 expression was up-regulated in the siliques at 4 days post anthesis (DPA). Confocal microscopy proved AtHsp90.5 was located in the chloroplasts. Plastid development in the AtHsp90.5 mutants and co-suppressed plants was seriously impaired, and few thylakoid membranes were observed, indicating the involvement of AtHsp90.5 in chloroplast biogenesis. AtHsp90.5 was found to interact with vesicle-inducing protein in plastids 1 (VIPP1) by bimolecular fluorescence complementation system. The ratio between VIPP1 oligomers and monomers in AtHsp90.5 co-suppressed plants drastically shifted toward the oligomeric state. Our study confirmed that AtHsp90.5 is vital for chloroplast biogenesis and embryogenesis. Further evidence also suggested that AtHsp90.5 may help in the disassembly of VIPP1 for thylakoid membrane formation and/or maintenance.
学科领域	Plant Sciences
DOI	10.1111/ppl.12083
收录类别	SCI
语种	英语
WOS关键词	VESICLE-INDUCING PROTEIN ; GENE-EXPRESSION ; MOLECULAR CHAPERONE ; CHLORATE-RESISTANT ; PHOTOSYSTEM-II ; IN-VIVO ; THALIANA ; REVEALS ; IMPORT ; IDENTIFICATION
WOS研究方向	Plant Sciences
WOS记录号	WOS:000329585700010
出版者	WILEY
文献子类	Article
出版地	HOBOKEN
EISSN	1399-3054
资助机构	National Natural Science Foundation of China [31200238] ; National High Technology and Research Development Program of China (863 project) [2007AA091705]
作者邮箱	yxli@ibcas.ac.cn
引用统计	被引频次：38[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27162
专题	中科院植物分子生理学重点实验室
作者单位	Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Feng, Juanjuan,Fan, Pengxiang,Jiang, Ping,et al. Chloroplast-targeted Hsp90 plays essential roles in plastid development and embryogenesis in Arabidopsis possibly linking with VIPP1[J]. PHYSIOLOGIA PLANTARUM,2014,150(2):292-307.
APA	Feng, Juanjuan,Fan, Pengxiang,Jiang, Ping,Lv, Sulian,Chen, Xianyang,&Li, Yinxin.(2014).Chloroplast-targeted Hsp90 plays essential roles in plastid development and embryogenesis in Arabidopsis possibly linking with VIPP1.PHYSIOLOGIA PLANTARUM,150(2),292-307.
MLA	Feng, Juanjuan,et al."Chloroplast-targeted Hsp90 plays essential roles in plastid development and embryogenesis in Arabidopsis possibly linking with VIPP1".PHYSIOLOGIA PLANTARUM 150.2(2014):292-307.

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