Knowledge Management System Of Institute Of Botany,CAS
| Discriminating plants using the DNA barcode rbcLb: an appraisal based on a large data set | |
| Dong, Wenpan; Cheng, Tao1; Li, Changhao1; Xu, Chao1; Long, Ping2; Chen, Chumming3; Zhou, Shiliang | |
| 2014 | |
| 发表期刊 | MOLECULAR ECOLOGY RESOURCES
 |
| ISSN | 1755-098X |
| 卷号 | 14期号:2页码:336-343 |
| 摘要 | The ideal DNA barcode for plants remains to be discovered, and the candidate barcode rbcL has been met with considerable skepticism since its proposal. In fact, the variability within this gene has never been fully explored across all plant groups from algae to flowering plants, and its performance as a barcode has not been adequately tested. By analysing all of the rbcL sequences currently available in GenBank, we attempted to determine how well a region of rbcL performs as a barcode in species discrimination. We found that the rbcLb region was more variable than the frequently used rbcLa region. Both universal and plant group-specific primers were designed to amplify rbcLb, and the performance of rbcLa and rbcLb was tested in several ways. Using blast, both regions successfully identified all families and nearly all genera; however, the successful species identification rates varied significantly among plant groups, ranging from 24.58% to 85.50% for rbcLa and from 36.67% to 90.89% for rbcLb. Successful species discrimination ranged from 5.19% to 96.33% for rbcLa and from 22.09% to 98.43% for rbcLb in species-rich families, and from 0 to 88.73% for rbcLa and from 2.04% to 100% for rbcLb in species-rich genera. Both regions performed better for lower plants than for higher plants, although rbcLb performed significantly better than rbcLa overall, particularly for angiosperms. Considering the applicability across plants, easy and unambiguous alignment, high primer universality, high sequence quality and high species discrimination power for lower plants, we suggest rbcLb as a universal plant barcode. |
| 关键词 | universal primers nucleotide diversity DNA barcode rbcLb |
| 学科领域 | Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology |
| DOI | 10.1111/1755-0998.12185 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS关键词 | MATK PRIMERS ; IDENTIFICATION ; SEQUENCES ; MARKERS ; SPACER |
| WOS研究方向 | Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology |
| WOS记录号 | WOS:000331469500010 |
| 出版者 | WILEY |
| 文献子类 | Article |
| 出版地 | HOBOKEN |
| EISSN | 1755-0998 |
| 资助机构 | Ministry of Science and Technology of China [2012BAC0 1B05-6, 2011FY120200, 2012AA021602] ; National Natural Science Foundation of China [31270239] |
| 作者邮箱 | slzhou@ibcas.ac.cn |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.ibcas.ac.cn/handle/2S10CLM1/27178 |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Baotou Med Coll, Baotou 014060, Peoples R China 4.Dr Cecilia Koo Bot Conservat & Environm Protect F, Pingtung 906, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Wenpan,Cheng, Tao,Li, Changhao,et al. Discriminating plants using the DNA barcode rbcLb: an appraisal based on a large data set[J]. MOLECULAR ECOLOGY RESOURCES,2014,14(2):336-343. |
| APA | Dong, Wenpan.,Cheng, Tao.,Li, Changhao.,Xu, Chao.,Long, Ping.,...&Zhou, Shiliang.(2014).Discriminating plants using the DNA barcode rbcLb: an appraisal based on a large data set.MOLECULAR ECOLOGY RESOURCES,14(2),336-343. |
| MLA | Dong, Wenpan,et al."Discriminating plants using the DNA barcode rbcLb: an appraisal based on a large data set".MOLECULAR ECOLOGY RESOURCES 14.2(2014):336-343. |
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| Dong-2014-Discrimina（179KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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