A systematic proteomic analysis of NaCl-stressed germinating maize seeds

doi:10.1007/s11033-014-3205-7

IB-CAS > 中科院光生物学重点实验室

	A systematic proteomic analysis of NaCl-stressed germinating maize seeds
	Meng, Ling-Bo ; Chen, Yi-Bo ; Lu, Tian-Cong ; Wang, Yue-Feng ; Qian, Chun-Rong ; Yu, Yang ; Ge, Xuan-Liang ; Li, Xiao-Hui ; Wang, Bai-Chen
	2014
发表期刊	MOLECULAR BIOLOGY REPORTS
ISSN	0301-4851
卷号	41 期号:5 页码:3431-3443
摘要	Salt (NaCl) is a common physiological stressor of plants. To better understand how germinating seeds respond to salt stress, we examined the changes that occurred in the proteome of maize seeds during NaCl-treated germination. Phenotypically, salt concentrations less than 0.2 M appear to delay germination, while higher concentrations disrupt development completely, leading to seed death. The identities of 96 proteins with expression levels altered by NaCl-incubation were established using 2-DE-MALDI-TOF-MS and 2-DE-MALDI-TOF-MS/MS. Of these 96 proteins, 79 were altered greater than twofold when incubated with a 0.2 M salt solution, while 51 were altered when incubated with a 0.1 M salt solution. According to their functional annotations in the Swiss-Prot protein-sequence databases, these proteins are mainly involved in seed storage, energy metabolism, stress response, and protein metabolism. Notably, the expression of proteins that respond to abscisic acid signals increased in response to salt stress. The results of this study provide important clues as to how NaCl stresses the physiology of germinating maize seeds.
关键词	Germination Maize embryo Salt stress Proteomics
学科领域	Biochemistry & Molecular Biology
DOI	10.1007/s11033-014-3205-7
收录类别	SCI
语种	英语
WOS关键词	GENE-EXPRESSION PROFILES ; ABSCISIC-ACID ; POSTTRANSLATIONAL MODIFICATIONS ; DORMANCY BREAKING ; ROOT INITIATION ; ALEURONE LAYER ; MESSENGER-RNAS ; BARLEY-SEEDS ; PROTEINS ; SALINITY
WOS研究方向	Biochemistry & Molecular Biology
WOS记录号	WOS:000335734400076
出版者	SPRINGER
文献子类	Article
出版地	DORDRECHT
EISSN	1573-4978
资助机构	National High-Tech Research and Development Program of China [2012AA10A300] ; Chinese Special Fund for Agro-scientific Research in the Public Interest [20093001-06-5]
作者邮箱	lixiaohui2002lix@163.com ; wangbc@ibcas.ac.cn
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ibcas.ac.cn/handle/2S10CLM1/27180
专题	中科院光生物学重点实验室
作者单位	1.Harbin Univ, Dept Life Sci, Harbin 150086, Peoples R China 2.Northeast Agr Univ, Sch Hort, Harbin 150030, Peoples R China 3.[Chen, Yi-Bo 4.Wang, Yue-Feng 5.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 6.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100093, Peoples R China 7.Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100093, Peoples R China 8.[Qian, Chun-Rong 9.Yu, Yang 10.Heilongjiang Acad Agr Sci, Inst Crop Cultivat & Farming, Harbin 150086, Peoples R China 11.Jilin Acad Agr Sci, Ctr Agribiotechnol, Changchun 130033, Peoples R China
推荐引用方式 GB/T 7714	Meng, Ling-Bo,Chen, Yi-Bo,Lu, Tian-Cong,et al. A systematic proteomic analysis of NaCl-stressed germinating maize seeds[J]. MOLECULAR BIOLOGY REPORTS,2014,41(5):3431-3443.
APA	Meng, Ling-Bo.,Chen, Yi-Bo.,Lu, Tian-Cong.,Wang, Yue-Feng.,Qian, Chun-Rong.,...&Wang, Bai-Chen.(2014).A systematic proteomic analysis of NaCl-stressed germinating maize seeds.MOLECULAR BIOLOGY REPORTS,41(5),3431-3443.
MLA	Meng, Ling-Bo,et al."A systematic proteomic analysis of NaCl-stressed germinating maize seeds".MOLECULAR BIOLOGY REPORTS 41.5(2014):3431-3443.

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